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transformation

  • Open Access
    Genetic Modification of <em>Sodalis</em> Species by DNA Transduction
    Research Article | Applied and Environmental Science
    Genetic Modification of Sodalis Species by DNA Transduction

    A large number of economically important insects maintain intimate associations with maternally inherited endosymbiotic bacteria. Due to the inherent nature of these associations, insect endosymbionts cannot be usually isolated in pure culture or genetically manipulated.

    Chelsea M. Keller, Christopher G. Kendra, Roberto E. Bruna, David Craft, Mauricio H. Pontes
  • Open Access
    Conjugal DNA Transfer in <span class="named-content genus-species" id="named-content-1">Sodalis glossinidius</span>, a Maternally Inherited Symbiont of Tsetse Flies
    Resource Report | Applied and Environmental Science
    Conjugal DNA Transfer in Sodalis glossinidius, a Maternally Inherited Symbiont of Tsetse Flies

    Tsetse flies are the insect vectors of T. brucei, the causative agent of African sleeping sickness—a zoonotic disease that inflicts a substantial economic cost on a broad region of sub-Saharan Africa. Notably, tsetse flies can be infected with the bacterium S. glossinidius to establish an asymptomatic chronic infection. This infection can be inherited by...

    Christopher G. Kendra, Chelsea M. Keller, Roberto E. Bruna, Mauricio H. Pontes
  • Open Access
    Development of a Plasmid Shuttle Vector System for Genetic Manipulation of <span class="named-content genus-species" id="named-content-1">Chlamydia psittaci</span>
    Research Article | Host-Microbe Biology
    Development of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci

    Psittacosis, caused by avian C. psittaci, has a major economic impact in the poultry industry worldwide and represents a significant risk for zoonotic transmission to humans. In the past decade, the tools of genetic manipulation have been improved for chlamydial molecular studies. While several genetic tools have been mainly developed in...

    Kensuke Shima, Mary M. Weber, Christiane Schnee, Konrad Sachse, Nadja Käding, Matthias Klinger, Jan Rupp
  • Open Access
    Factors and Conditions That Impact Electroporation of <span class="named-content genus-species" id="named-content-1">Clostridioides difficile</span> Strains
    Research Article | Molecular Biology and Physiology
    Factors and Conditions That Impact Electroporation of Clostridioides difficile Strains

    Understanding the underlying biology of pathogens is essential to develop novel treatment options. To drive this understanding, genetic tools are essential. In recent years, the genetic toolbox available to Clostridioides difficile researchers has expanded significantly but still requires the conjugal transfer of DNA from a donor strain into...

    Disha Bhattacharjee, Joseph A. Sorg
  • Open Access
    The Genetic Transformation of <span class="named-content genus-species" id="named-content-1">Chlamydia pneumoniae</span>
    Research Article | Host-Microbe Biology
    The Genetic Transformation of Chlamydia pneumoniae

    The absence of tools for the genetic manipulation of C. pneumoniae has hampered research into all aspects of its biology. In this study, we established a novel reproducible method for C. pneumoniae transformation based on a plasmid shuttle vector system. We constructed a...

    Kensuke Shima, Maximilian Wanker, Rachel J. Skilton, Lesley T. Cutcliffe, Christiane Schnee, Thomas A. Kohl, Stefan Niemann, Javier Geijo, Matthias Klinger, Peter Timms, Thomas Rattei, Konrad Sachse, Ian N. Clarke, Jan Rupp
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