transformation

  • Open Access
    Factors and Conditions That Impact Electroporation of <span class="named-content genus-species" id="named-content-1">Clostridioides difficile</span> Strains
    Research Article | Molecular Biology and Physiology
    Factors and Conditions That Impact Electroporation of Clostridioides difficile Strains

    Understanding the underlying biology of pathogens is essential to develop novel treatment options. To drive this understanding, genetic tools are essential. In recent years, the genetic toolbox available to Clostridioides difficile researchers has expanded significantly but still requires the conjugal transfer of DNA from a donor strain into...

    Disha Bhattacharjee, Joseph A. Sorg
  • Open Access
    The Genetic Transformation of <span class="named-content genus-species" id="named-content-1">Chlamydia pneumoniae</span>
    Research Article | Host-Microbe Biology
    The Genetic Transformation of Chlamydia pneumoniae

    The absence of tools for the genetic manipulation of C. pneumoniae has hampered research into all aspects of its biology. In this study, we established a novel reproducible method for C. pneumoniae transformation based on a plasmid shuttle vector system. We constructed a...

    Kensuke Shima, Maximilian Wanker, Rachel J. Skilton, Lesley T. Cutcliffe, Christiane Schnee, Thomas A. Kohl, Stefan Niemann, Javier Geijo, Matthias Klinger, Peter Timms, Thomas Rattei, Konrad Sachse, Ian N. Clarke, Jan Rupp
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