plasmid shuttle vector

  • Open Access
    Development of a Plasmid Shuttle Vector System for Genetic Manipulation of <span class="named-content genus-species" id="named-content-1">Chlamydia psittaci</span>
    Research Article | Host-Microbe Biology
    Development of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci

    Psittacosis, caused by avian C. psittaci, has a major economic impact in the poultry industry worldwide and represents a significant risk for zoonotic transmission to humans. In the past decade, the tools of genetic manipulation have been improved for chlamydial molecular studies. While several genetic tools have been mainly developed in...

    Kensuke Shima, Mary M. Weber, Christiane Schnee, Konrad Sachse, Nadja Käding, Matthias Klinger, Jan Rupp
  • Open Access
    The Genetic Transformation of <span class="named-content genus-species" id="named-content-1">Chlamydia pneumoniae</span>
    Research Article | Host-Microbe Biology
    The Genetic Transformation of Chlamydia pneumoniae

    The absence of tools for the genetic manipulation of C. pneumoniae has hampered research into all aspects of its biology. In this study, we established a novel reproducible method for C. pneumoniae transformation based on a plasmid shuttle vector system. We constructed a...

    Kensuke Shima, Maximilian Wanker, Rachel J. Skilton, Lesley T. Cutcliffe, Christiane Schnee, Thomas A. Kohl, Stefan Niemann, Javier Geijo, Matthias Klinger, Peter Timms, Thomas Rattei, Konrad Sachse, Ian N. Clarke, Jan Rupp
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