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    • Latest Articles
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    • mSphere of Influence: Commentaries from Early Career Microbiologists
    • Archive
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intracellular parasites

  • Open Access
    mSphere of Influence: Finding a Direction—How Do Mitochondria Know Where To Go?
    Commentary | Host-Microbe Biology
    mSphere of Influence: Finding a Direction—How Do Mitochondria Know Where To Go?

    Lena Pernas works in the field of metabolism of infection. In this mSphere of Influence article, she reflects on how work by Brian Cunniff (B. Cunniff, A. J. McKenzie, N. H. Heintz, and A. K. Howe, Mol Biol Cell 27:2662–2674, 2016, https://doi.org/10.1091/mbc.e16-05-0286) and Thomas Schwarz (G. Pekkurnaz, J. C. Trinidad, X. Wang, D. Kong, and T. L. Schwarz, Cell 158:54–68, 2014,...

    Lena Pernas
  • Open Access
    Monoclonal Antibodies to Intracellular Stages of <span class="named-content genus-species" id="named-content-1">Cryptosporidium parvum</span> Define Life Cycle Progression <em>In Vitro</em>
    Research Article | Host-Microbe Biology
    Monoclonal Antibodies to Intracellular Stages of Cryptosporidium parvum Define Life Cycle Progression In Vitro

    Cryptosporidium is a protozoan parasite that causes gastrointestinal disease in humans and animals. Currently, there is a limited array of antibodies available against the parasite, which hinders imaging studies and makes it difficult to visualize the parasite life cycle in different culture systems. In order to alleviate this reagent gap, we created a library of novel antibodies against the intracellular life cycle stages of...

    Georgia Wilke, Soumya Ravindran, Lisa Funkhouser-Jones, Jennifer Barks, Qiuling Wang, Kelli L. VanDussen, Thaddeus S. Stappenbeck, Theresa B. Kuhlenschmidt, Mark S. Kuhlenschmidt, L. David Sibley
  • Open Access
    Deep Sequencing of RNA from Blood and Oral Swab Samples Reveals the Presence of Nucleic Acid from a Number of Pathogens in Patients with Acute Ebola Virus Disease and Is Consistent with Bacterial Translocation across the Gut
    Research Article | Host-Microbe Biology
    Deep Sequencing of RNA from Blood and Oral Swab Samples Reveals the Presence of Nucleic Acid from a Number of Pathogens in Patients with Acute Ebola Virus Disease and Is Consistent with Bacterial Translocation across the Gut

    Our results highlight the identification of an array of pathogens in the blood of patients with Ebola virus disease (EVD). This has not been done before, and the data have important implications for the treatment of patients with EVD, particularly considering antibiotic stewardship. We show that EVD patients who were also infected with Plasmodium, particularly at higher loads, had more adverse outcomes than patients with lower...

    Miles W. Carroll, Sam Haldenby, Natasha Y. Rickett, Bernadett Pályi, Isabel Garcia-Dorival, Xuan Liu, Gary Barker, Joseph Akoi Bore, Fara Raymond Koundouno, E. Diane Williamson, Thomas R. Laws, Romy Kerber, Daouda Sissoko, Nóra Magyar, Antonino Di Caro, Mirella Biava, Tom E. Fletcher, Armand Sprecher, Lisa F. P. Ng, Laurent Rénia, N’faly Magassouba, Stephan Günther, Roman Wölfel, Kilian Stoecker, David A. Matthews, Julian A. Hiscox
  • Open Access
    
<span class="named-content genus-species" id="named-content-1">Toxoplasma gondii</span> AP2IX-4 Regulates Gene Expression during Bradyzoite Development
    Research Article | Host-Microbe Biology
    Toxoplasma gondii AP2IX-4 Regulates Gene Expression during Bradyzoite Development

    Toxoplasma gondii is a single-celled parasite that persists in its host as a transmissible tissue cyst. How the parasite converts from its replicative form to the bradyzoites housed in tissue cysts is not well understood, but the process clearly involves changes in gene expression. Here we report that parasites lacking a cell cycle-regulated transcription factor...

    Sherri Huang, Michael J. Holmes, Joshua B. Radke, Dong-Pyo Hong, Ting-Kai Liu, Michael W. White, William J. Sullivan
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