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IgG1

  • Open Access
    Assignment of Serotype-Specific IgG1, IgG2, and IgA Weight-Based Antibody Units to the Human Pneumococcal Standard Reference Serum, 007sp
    Research Article | Clinical Science and Epidemiology
    Assignment of Serotype-Specific IgG1, IgG2, and IgA Weight-Based Antibody Units to the Human Pneumococcal Standard Reference Serum, 007sp

    A well-characterized antibody standard is an indispensable reagent for use in assays designed to measure antibodies with precision and where assays between laboratories need to be comparable. The human pneumococcal standard reference serum, lot 89SF, greatly facilitated the standardization of enzyme-linked immunosorbent assay methodologies during a critical period when the first pneumococcal polysaccharide-conjugate vaccines were being...

    Scott Jones, Kier Finnegan, Jae Hee Wee, Polly D’Argoeuves, Lucy Roalfe, Suzanne Byrne, Sarah Heffernan, Adam Hunt, Marina Johnson, Tessa Jones, Emma Pearce, Hayley Richardson, Vasili Thalasselis, David Goldblatt
  • Open Access
    Mixed <span class="named-content genus-species" id="named-content-1">Chlamydia trachomatis</span> Peptide Antigens Provide a Specific and Sensitive Single-Well Colorimetric Enzyme-Linked Immunosorbent Assay for Detection of Human Anti<em>-</em><span class="named-content genus-species" id="named-content-2">C. trachomatis</span> Antibodies
    Research Article | Clinical Science and Epidemiology
    Mixed Chlamydia trachomatis Peptide Antigens Provide a Specific and Sensitive Single-Well Colorimetric Enzyme-Linked Immunosorbent Assay for Detection of Human Anti-C. trachomatis Antibodies

    For detection of anti-C. trachomatis antibodies by serological assays, use of classical chlamydial antigens results in high cross-reactivity and poor sensitivity. Previously, we discovered 48 strongly reactive peptide antigens of C. trachomatis-specific B-cell epitopes from 21 immunodominant...

    K. Shamsur Rahman, Toni Darville, Harold C. Wiesenfeld, Sharon L. Hillier, Bernhard Kaltenboeck
  • Open Access
    Comprehensive Molecular Serology of Human <em>Chlamydia trachomatis</em> Infections by Peptide Enzyme-Linked Immunosorbent Assays
    Research Article | Clinical Science and Epidemiology
    Comprehensive Molecular Serology of Human Chlamydia trachomatis Infections by Peptide Enzyme-Linked Immunosorbent Assays

    For detection of anti-Chlamydia trachomatis antibodies by serological assays, use of classical whole-organism chlamydial antigens results in high cross-reactivity. These antigens bind mainly antibodies against the major outer membrane protein (OmpA) and bind antibodies against other immunodominant non-OmpA proteins to a lesser extent, resulting in poor assay...

    K. Shamsur Rahman, Toni Darville, Ali N. Russell, Catherine M. O'Connell, Harold C. Wiesenfeld, Sharon L. Hillier, De’Ashia E. Lee, Bernhard Kaltenboeck
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