cell biology
Research Article | Host-Microbe BiologyTetanus Toxin cis-Loop Contributes to Light-Chain TranslocationHow protein toxins translocate their catalytic domain across a cell membrane is the least understood step in toxin action. This study utilized a reporter, β-lactamase, that was genetically fused to full-length, nontoxic tetanus toxin (βlac-TT) in discovery-based live-cell assays to study LC translocation. Directed mutagenesis identified a role for K768 in LC translocation. K768 was located between α15 and α16 (...
Editor's Pick Research Article | Therapeutics and PreventionEssential Assembly Factor Rpf2 Forms Novel Interactions within the 5S RNP in Trypanosoma bruceiTrypanosoma brucei is the parasitic protozoan that causes African sleeping sickness. Ribosome assembly is essential for the survival of this parasite through the different host environments it encounters during its life cycle. The assembly of the 5S ribonucleoprotein particle (5S RNP) functions as one of the regulatory checkpoints during ribosome biogenesis. We have...