Candida albicans
Research Article | Molecular Biology and PhysiologyGenetic Analysis of NDT80 Family Transcription Factors in Candida albicans Using New CRISPR-Cas9 ApproachesTranscription factors play key roles in regulating virulence of the human fungal pathogen C. albicans. In addition to regulating the expression of virulence factors, they also control the ability of C. albicans to switch to filamentous hyphal growth, which facilitates biofilm formation on medical...
Research Article | Therapeutics and PreventionAlexidine Dihydrochloride Has Broad-Spectrum Activities against Diverse Fungal PathogensThe prevalence of fungal infections has seen a rise in the past decades due to advances in modern medicine leading to an expanding population of device-associated and immunocompromised patients. Furthermore, the spectrum of pathogenic fungi has changed, with the emergence of multidrug-resistant strains such as C. auris. High mortality related to fungal infections...
Research Article | Molecular Biology and PhysiologyFunctional Mapping of Transcription Factor Grf10 That Regulates Adenine-Responsive and Filamentation Genes in Candida albicansMetabolic adaptation and morphogenesis are essential for Candida albicans, a major human fungal pathogen, to survive and infect diverse body sites in the mammalian host. C. albicans utilizes transcription factors to tightly control the transcription of metabolic genes and morphogenesis genes. Grf10...
Resource Report | Molecular Biology and PhysiologyA High-Throughput Candida albicans Two-Hybrid SystemCandida albicans is a major fungal pathogen, and due to the rise of fungal infections and emerging resistance to the limited antifungals available, it is important to develop novel and more specific antifungals. Protein-protein interactions (PPIs) can be applied as very specific drug targets. However, because of the aberrant codon usage of...
Research Article | Molecular Biology and PhysiologyMultiple Evolutionarily Conserved Domains of Cap2 Are Required for Promoter Recruitment and Iron Homeostasis Gene RegulationIron is an essential micronutrient for living cells. Candida albicans, the predominant human fungal pathogen, thrives under diverse environments with vastly different iron levels in the mammalian host. Therefore, to tightly control iron homeostasis, C. albicans has evolved a set of transcriptional...
Research Article | Molecular Biology and PhysiologyThe 5′ Untranslated Region of the EFG1 Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in Candida albicansMany of the virulence traits that make Candida albicans an important human fungal pathogen are regulated on a transcriptional level. Here, we report an important regulatory contribution of translation, which is exerted by the extensive 5′ untranslated regulatory sequence (5′ UTR) of the transcript for the protein Efg1, which determines growth, metabolism, and...
Research Article | Molecular Biology and PhysiologyRapid Gene Concatenation for Genetic Rescue of Multigene Mutants in Candida albicansOur understanding of new genes is often built upon the knowledge of well-characterized genes. One avenue toward revealing such connections involves creation of strains with mutations in two or more defined genes to permit genetic interaction analysis. Strain manipulations can yield unexpected mutations at loci outside the defined targeted genes. In this report, we describe a method for rapid validation of multigene mutants, thus...
Research Article | Therapeutics and PreventionNovel Antifungal Compounds Discovered in Medicines for Malaria Venture’s Malaria BoxMuch like the recent increase in drug-resistant bacteria, there is a rise in antifungal-resistant strains of pathogenic fungi. There is a need for novel and more potent antifungal therapeutics. Consequently, we investigated a mixed library of drug-like and probe-like compounds with activity in Plasmodium spp. for activity against two common fungal pathogens, ...
Research Article | Molecular Biology and PhysiologyPut3 Positively Regulates Proline Utilization in Candida albicansCandida albicans poses a significant threat to the lives of immunocompromised people. Historically, knowledge has been drawn from studies on Saccharomyces cerevisiae to understand the pathogen, and many Candida albicans genes are...
Editor's Pick Research Article | Host-Microbe BiologyThe Human Gut Microbial Metabolome Modulates Fungal Growth via the TOR Signaling PathwayCandida albicans is a natural component of the human microbiota but also an opportunistic pathogen that causes life-threatening infections. The human gastrointestinal tract is the main reservoir of C. albicans, from where systemic infections originate as a consequence of the disruption of the...