APEX
Research Article | Molecular Biology and PhysiologyProximity Staining Using Enzymatic Protein Tagging in DiplomonadsThe function of many proteins is intrinsically related to their cellular location. Novel methods for ascertainment of the ultrastructural location of proteins have been introduced in recent years, but their implementation in protists has so far not been readily realized. Here, we present an optimized proximity labeling protocol using the APEX system in the salmon pathogen ...
Research Article | Molecular Biology and PhysiologyComparative Cell Biology and Evolution of Annexins in DiplomonadsAnnexins are proteins that associate with phospholipids in a Ca2+-dependent fashion. These proteins have been intensely studied in animals and plants because of their importance in diverse cellular processes, yet very little is known about annexins in single-celled eukaryotes, which represent the largest diversity of organisms. The human intestinal parasite ...