TABLE 3

Strains and plasmids used in this study

Strain or plasmidGenotype or descriptionaReference or source
Strains
 A. baumannii
        ATCC 17978Noninternational type clone (wild type)ATCC (70)
        ΔqseBCATCC 17978 with Eryr insertion disruption in qseBCThis study
        ΔygiWATCC 17978 with Eryr insertion disruption in ygiWThis study
        ΔhnsATCC 17978 with hns disrupted by ISAba1227
        Δhns::ISAba12ATCC 17978 with hns disrupted by ISAba12This study
        ΔqseBC Δhns::ISAba12ΔqseBC with hns disrupted by ISAba12This study
        ΔygiW Δhns::ISAba12ΔygiW with hns disrupted by ISAba12This study
        ΔygiW Δhns::MITEAba12ΔygiW with hns disrupted by MITEAba12This study
        Δhns pWH0268Δhns with pWH026827
        Δhns::ISAba12 pWH0268Δhns::ISAba12 with pWH0268This study
        ΔqseBC Δhns::ISAba12 pWH0268ΔqseBC Δhns::ISAba12 with pWH0268This study
        ΔygiW Δhns::ISAba12 pWH0268ΔygiW Δhns::ISAba12 with pWH0268This study
        ΔygiW Δhns::MITEAba12 pWH0268ΔygiW Δhns::MITEAba12 with pWH0268This study
 E. coli
        DH5α λpirF Φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK, mK+)
phoA supE44 λ thi-1 gyrA96 relA1 λpir,
conjugative strain which can host λ-pir-dependent plasmids
87
Plasmids
    pAT04Tetr; pMMB67EH with RecAb system71
    pGEM-T EasyAmpr; T-overhang cloning vectorPromega
    pVA891Cmlr Eryr; Source of Eryr cassette88
    pWH0268Ampr; pWH1266 with hns cloned via BamHI restriction site27
  • a Abbreviations: Amp, ampicillin; Cml, chloramphenicol; Ery, erythromycin; Tet, tetracycline.