TABLE 1

Bacterial strains used in this study and their MIC values obtained using the Etesta

Strain or plasmidDescriptionDAP MIC
(µg/ml)
Reference
or source
Strains
    S. aureus
        N315Hospital-acquired
methicillin-resistant
SCCmec type II
0.12560
        ATCC 29213MSSA, standard strain
for CLSI antimicrobial
susceptibility testing
0.12561
        NewmanMSSA, isolated from
a human infection
0.2562
        KVRN315 ΔvraSR::cat60
        CB1631DAPs, SCCmec type II0.2541
        CB1634DAPr isogenic to CB1631441
        CB1634+agrCB1634 + expressing
psk265 full-length agr
0.094This study
        CB5013DAPs, SCCmec type II0.2541
        CB5014DAPr, isogenic to CB5013441
        CB1634ΔvraSRCB1634 ΔvraSR::cat0.25This study
        CB1634ΔvraSRvraSRCB1634 ΔvraSR::cat
pVRASR-2
2This study
        CB5014ΔvraSRCB5014 ΔvraSR::cat0.25This study
        CB5014ΔvraSRvraSRCB5014 ΔvraSR::cat
pVRASR-2
2This study
    S. epidermidis Y10.25This study
Plasmids
    pCR-XL-2 TOPOCloning vector,
Ampr Kanr
ThermoFisher
    S. aureus RN4220(pVRASR-2)Entire vraS/vraR
cloned into pAW8-tet
39
  • a Abbreviations: cat, chloramphenicol resistant; Tetr, tetracycline resistant; Ampr, ampicillin resistant; Kanr, kanamycin resistant.