TABLE 1

Bacterial strains and plasmids

Strain or
plasmid
Genotype or descriptionReference or
source
Strains
    E. coli S17-1recA thi pro hsdR RP4-2-Tc::Mu-Km::Tn7λpir Smr Tpr53
    P. fluorescens
    Pf0-1Wild type, Apr54
    Pf0-ecfpPf0-1::mini-Tn7 ecfp GmrThis study
    Pedobacter
    V48Wild type55
    V48-dsRedV48 N824_RS25465T899::HimarEm1 PompA-dsRedEXPRESS Emr;
NCBI accession no. NZ_AWRU00000000.1, as of 25 September 2017
This study
Plasmids
    pUC18T-mini-Tn7T-Gm-dsRedEXPRESSGmr21
    pUC18T-mini-Tn7T-Gm-ecfpGmr21
    pUX-BF13R6K replicon-based helper plasmid carrying Tn7 transposase genes56
    pHimarEm1Plasmid carrying HimarEm1; Kmr (Emr)23
    pHimarEm1-dsRedpHimarEm1Ω(3.529 kb::PompA-dsRedEXPRESS)This study