TABLE 2

Prevalence of the investigated ExPEC-associated genes

Category and geneaDescriptionPrevalence (%)GenBank accession no.
APEC
(n = 32)
ExPEC
(n = 291)
A/E-PEC
(n = 127)
HExPEC-1
(n = 147)
HExPEC-2
(n = 49)
A/E-PEC-associated genes
    iucC1Aerobactin synthesis96.935.485.414.54.2AAS66995.1
    iucD1Aerobactin synthesis96.935.486.213.84.2AAA23196.1
    iutA1Iron transport96.935.184.614.54.2AAS66997.1
    cvaA1Colicin V96.933.083.113.10.0CAA11514.1
    etsA1ABC transport system96.930.677.713.10.0YP_444079.1
    hlyF1Hemolysin93.834.485.413.10.0AAO49613.1
    ompT1/2Outer membrane protease93.834.084.613.10.0ADK70174.1
    cvaB1Colicin V87.533.381.513.10.0CAA11515.1
    cvaC1Colicin V84.432.378.513.10.0CAA40746.1
    cvi1Colicin V immunity84.433.380.813.10.0CAA11513.1
HExPEC-1- and A/E-PEC-
associated genes
    tia2Invasion determinant protein71.975.684.691.70.0ABJ02397.1
    papE2P fimbria71.975.383.891.70.0CAA43568.1
    ireA2Siderphore receptor71.974.983.191.70.0AMR36194.1
    papG-II2P fimbria adhesin variant71.974.983.891.00.0AAA24293.1
    papA2P fimbria shaft68.874.681.591.70.0AAZ04426.1
HExPEC-2-associated genes
    ibeA2Brain epithelium invasion40.621.320.00.7100AAA92443.1
    sfaS2S fimbria adhesin28.119.613.80.797.9AAB25046.1
    sfaG2S fimbria28.118.611.50.797.9AAB25045.1
    cnf12Cytotoxic necrotizing factor0.017.23.10.793.8CAA50007.1
    papG-III2P fimbria adhesin variant0.015.80.80.093.8WP_001468556.1
HExPEC-2- and A/E-PEC-
associated gene
    iroN1Siderophore96.949.183.113.197.9AAN76093.1
Highly frequent (genes present
in >75% of all strains)
    csgA1/2Curli100100100100100AAA23616.1
    fyuA2Siderophore100100100100100CAA86211.1
    ompA2Serum resistance100100100100100AAN79561.1
    iss1Serum survival100100100100100X52665
    feoB3Iron uptake10099.710099.3100NP_417868.1
    fimH2Type 1 fimbria adhesin10099.710099.3100CAA12423.1
    usp2Uropathogenic-specific protein (bacteriocin)10099.399.299.3100BAA93674.1
    irp22Yersiniabactin10098.698.598.6100OAC27446.1
    kpsMT-K12Group 2 capsule kpsM variant10099.799.2100100AAA24046.1
    malX (PAI)2Pathogenicity island81.398.391.5100100AF003742.1
    papC2P fimbria usher71.991.184.691.095.8CAA43564.1
    papF2P fimbria subunit71.991.485.491.793.8AAZ04419.1
    vat2Vacuolating autotransporter toxin59.488.080.096.664.6AAO21903.1
    bor3Serum resistance96.977.374.688.364.6AGD80583.1
    sitA2Iron transport53.178.054.688.393.8CAJ21596.1
Genes of variable presence
    traT1Serum resistance43.857.450.066.939.6AAA26075.1
    eitA1Iron ABC transport system31.311.710.89.733.3ABD51672.1
    cma1Colicin M34.42.413.10.70.0AFK30933.1
    cbi1Colicin B immunity31.32.713.10.70.0YP_003937726.1
    cdtB2Cytolethal distending toxin B0.05.812.30.02.1BAL47227.1
Low frequency (genes identified in <5% of all investigated genomes)
    astA2EAST1 toxin12.51.04.60.70.0AAG18472.1
    tsh1Hemagglutinin12.50.03.10.00.0AAA24698.1
    iha1Nonfimbrial Iha adhesin0.00.30.00.70.0ABB17254.1
    focG2F1C fimbria0.00.30.80.00.0AAB20438.1
    afa2Nonfimbrial adhesin0.00.00.00.00.0CAA54113.1
    fliC-H72Flagellin0.00.00.00.00.0AGA03821.1
    hlyD2α-Hemolysin0.00.00.00.00.0YP_002756568.1
    papG-I2P fimbria adhesin variant0.00.00.00.00.0CAA43570.1
    bfp1Bundle-forming pilus0.00.00.00.00.0AID54475.1
    bmaBlood group M adhesin0.00.00.00.00.0AAA23523.1
    gafDG-fimbriae0.00.00.00.00.0AAA69514.1
    rfc2Lipopolysaccharide synthesis0.00.00.00.00.0AAC43898.1
    stgA2Stg fimbria0.00.00.00.00.0AAS99229.1
    stx2Shiga toxin0.00.00.00.00.0AB012101.1
  • a The category is the gene location or gene frequency class. Genes localized on plasmid are indicated by superscript 1, genes localized on chromosome are indicated by superscript 2, and genes carried on phage are indicated by superscript 3.