TABLE 2

Primers used in this study for quantitative PCR to confirm Gallus gallus genes as potential transformation markers

NCBI
reference
sequence
Gene namePrimer sequenceaProduct
size (bp)
NM_205041.12'-5′-Oligoadenylate synthetase like (OASL)For: 5′-AGGTCCTGGTGAAGGACAGT-3′145
Rev: 5′-TCCAGCTCCTTGGTCTCGTA-3′
28S RNAFor: 5′-GGTATGGGCCCGACGCT-3′144
Rev: 5′-CCGATGCCGACGCTCAT-3′
NM_205518.1Actin, beta (ACTB)For: 5′-GAGAAATTGTGCGTGACATCA-3′152
Rev: 5′-CCTGAACCTCTCATTGCCA-3′
NM_001031451.1FYN binding protein (FYB)For: 5′-GCCCCAAAACGGAAGTCTTTGC-3′256
Rev: 5′-TGGGCTTGACATTTCTGGGCG-3′
XM_001231970.4Glutathione S-transferase theta 1-like (GSTT1L)For: 5′-AACAGGCCAGGGTTGATGAG-3′137
Rev: 5′-AGCACCTTCCACTTTCTCCG-3′
NM_204305.1Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)For: 5′-AATGGCTTTCCGTGTGCCAACC-3′223
Rev: 5′-ATTCAGTGCAATGCCAGCACCC-3′
NM_001030753.1H2A histone family, member J (H2AFJ)For: 5′-AGGCCAAGTCGCGTTCATC-3′148
Rev: 5′-ATCTCGGCCGTCAGGTACT-3′
NM_001012576.1Heat shock protein family A (Hsp70) member 4-like (HSPA4L)For: 5′-TGGCGACAACTCCAAAGTGA-3′129
Rev: 5′-TCAGTATCCATCGCTGCGTC-3′
XM_418246.5Interferon, gamma-inducible protein 30 (IFI30)For: 5′-CGCTCAGGAGAGGAATGTCT-3′181
Rev: 5′-GCAAGCCTTCAGATTCTTGG-3′
NM_205342.1Lamin B receptor (LBR)For: 5′-GCAAACAAGATGACCCCAGC-3′150
Rev: 5′-GGCCTTCCACAACCTTTCCT-3′
M75729.1MDV 175-kDa protein (ICP4) geneFor: 5-TTTCTAGCAAGGAGCGACGC-3′81
Rev: 5-CTGACTTGCGCTTACGGGAA-3′
XM_015296999.1Regulator of chromosome condensation 2 (RCC2)For: 5′-CTGGTTGTAGGCTTGGAGCA-3′146
Rev: 5′-TGAGGAAGGAGGGTGGGAAA-3′
NM_001135968.1Transporter 1, ATP-binding cassette, subfamily B (MDR/TAP) (TAP1)For: 5′-ACGACTTCATCACTCGCCTGC-3′280
Rev: 5′-TCCAACACCACCACTCGTTGTG-3′
  • a For, forward primer; Rev, reverse primer.