TABLE 1

Impact of treatment and depth on environmental and chemical factors and on the bacterial communitya

Factor or bacterial familybBetween depthsWithin depths
TreatmentDepthTreatmentTreatment × depth
Light0.012<0.0010.0250.169
Temperature0.5550.0050.0030.547
Oxygen0.3090.0120.3460.014
Methane0.012<0.0010.0230.169
Methylococcaceae
    DNA0.2920.0330.0090.121
    RNA0.0940.0120.2770.259
Comamonadaceae
    DNA0.3640.0670.0050.114
    RNA0.1130.023<0.0010.970
Flavobacteriaceae
    DNA0.0400.0040.6530.995
    RNA0.2400.0730.6150.093
Chlorobiaceae
    DNA0.1810.0340.6800.321
    RNA0.0840.0140.6350.021
  • a The results of repeated measures two-way ANOVA for testing the impact of treatment and depth to light intensity, temperature, concentration of oxygen and methane, and the relative abundance of dominant taxonomic bacterial groups in the lake water column. Significant results (P < 0.05) are shown in bold type, while marginal significant results (P < 0.1) are shown in italic type.

  • b The dominant bacterial group or family and whether the bacterial community analysis was based on DNA or RNA are shown.