TABLE 1

Bacterial strains and plasmid used in this study

Bacterial strainRelevant featuresaReference
or source
B. pseudomallei K96243Human clinical isolate from Thailand; Ipms Cazs Chls Cips, Augmentins Minr Genr Tmp-Smxr Strr56
B. pseudomallei PBK001B. pseudomallei CLH010 with an unmarked intragenic deletion in BPSS0368 (ΔtonB)This study
B. pseudomallei CLH010B. pseudomallei K96243 with an unmarked intragenic deletion in BPSS1498 (Δhcp1)This study
E. coli S17-1 λpir
(pMo130-ΔBPSS1498)
Donor strain containing pMo130-Δbpss1498 plasmid; Smr Tpr Pbs Kmr23
  • a Ipm, imipenem; Caz, ceftazidime; Chl, chloramphenicol; Cip, ciprofloxacin; Augmentin, amoxicillin/clavulanate potassium; Min, minocycline; Gen, gentamicin; Tmp-Smx, trimethoprim-sulfamethoxazole; Str, streptomycin; Km, kanamycin; Pb, polymyxin B; Sm, streptomycin; Tp, trimethoprim.