TABLE 4

Definitions of meningococcal categories used in PCR analysesa

CategoryIsolate PCRDirect swab PCRWGS
All meningococciAny sample in which the porA and/or ctrA gene(s) was detectedAny sample in which the porA and/or ctrA gene(s) and/or the group B capsule gene was detectedAny sample in which the porA and/or ctrA sequences were predicted to encode full-length gene product
Grouped meningococciAny sample in which the porA and/or ctrA gene(s) and the relevant group capsule gene were detectedPerformed only for NmBAny sample in which the porA and/or ctrA sequences and all the required genes for synthesis and transport of the relevant capsule group were predicted to encode full-length gene products
Nongroupable meningococciAny sample in which the porA and/or ctrA gene(s) was detected but no capsule gene was detectedNot applicableAny sample in which the porA and ctrA sequences were predicted to encode full-length gene product and lacking any of the required genes for synthesis and transport of the relevant capsule group OR any sample in which the ctrA sequence is incomplete
Non-NmB meningococciNot applicableAny sample in which the porA and/or ctrA gene(s) was detected but the group B capsule gene was not detectedNot applicable
Group B meningococciSee “Grouped meningococci” aboveAny sample in which the group B capsule gene was detectedSee “Grouped meningococci” above
  • a Abbreviations: NmB, Neisseria meningitidis serogroup B; WGS, whole-genome sequencing.