TABLE 1

Bacterial experimental strains and plasmids

Species
and strain
PlasmidRelevant resistance
characteristic(s) [gene(s)]a
Relevant descriptionType of
sequence data
ReferenceGenBank
accession no.
E. faecium strains
    UW1551ECC-containing clinical isolate52
    UWΔpUW1551 cured of most plasmidsThis study
    UWECC::catChlr [cat]Plasmid-cured UW1551 with cat
resistance marker inserted in ORF1
next to ccrABEnt
This study
    K60-19reppLG1Genr [aac(6′)Ie-aph(2'')Ia]Clinical reppLG1 plasmid donor
with many other plasmids
23
    BM4105-RF reppLG1reppLG1Rifr, Fusr, Genr [aac(6′)Ie-aph(2'')Ia]reppLG1 plasmid donor containing
only this plasmid
This study
    UWECC::cat reppLG1reppLG1Chlr [cat], Genr [aac(6′)Ie-aph(2'')Ia]Donor UWECC::cat with reppLG1PacBioThis studyNMZL01000001.1,
NMZL01000002.1,
NMZL01000003.1
    BM4105-RFRifr, FusrRecipientNanopore and
Illumina
combined
54CP030110.1
    BMECC::catreppLG1Chlr [cat], Rifr, Fusr, Genr
[aac(6′)Ie-aph(2'')Ia]
Transconjugant containing ECC::cat
on BM4105-RF chromosome
PacBioThis studyNMZK01000001.1,
NMZK01000002.1
    BM pECC::catreppLG1ECC::catChlr [cat), Rifr, Fusr, Genr
[aac(6′)Ie-aph(2'')Ia]
Transconjugant containing ECC::cat
on plasmid
PacBioThis studyNMZJ01000001.1,
NMZJ01000002.1
E. coli strains
    pTEX5500tsChlr [cat], Genr [aph(2'')-Id]Shuttle plasmid, temperature sensitive
in Gram-positive host
53
    pORF1aChlr [cat], Genr [aph(2'')-Id]pTEX5500ts with cloned ORF1 fragment
upstream of the cat gene
This study
    pORF1bChlr [cat], Genr [aph(2'')-Id]pTEX5500ts with cloned ORF1 fragments
flanking the cat gene
This study
  • a The r superscript indicates resistance. Drugs are abbreviated as follows: Chl, chloramphenicol; Gen, gentamicin; Rif, rifampin; Fus, fusidic acid.