TABLE 1

GAS strains used in this study

GAS strainDescriptionReference
MGAS2096A serotype M12 GAS strain that was isolated from a patient with acute poststreptococcal
glomerulonephritis in 1960. Contains a 1-bp insertion in rocA
39
MGAS9429A serotype M12 GAS strain that was isolated from a pediatric patient with pharyngitis
in 2001. Contains a functional rocA allele
39
MGAS2221A serotype M1 GAS strain that has been extensively characterized14
2221ΔrocAMGAS2221 derivative in which the rocA gene has been replaced with a spectinomycin
resistance cassette
38
2096 pDCBBMGAS2096 derivative containing the chloramphenicol-resistant empty vector pDCBBThis study
2096 pRocAMGAS2096 derivative containing the chloramphenicol-resistant pRocA which expresses
functional RocA (as found in the M1 GAS strain MGAS2221)
This study
2096 pRocA-M18MGAS2096 derivative containing the chloramphenicol-resistant pRocA-M18 which
expresses a nonfunctional RocA (as found in M18 GAS)
This study