TABLE 5

ASV distribution of the gDNA mock community following different sequencing methodsa

TaxonomyNo. (%) of OTUs/ASVs byb:
IlluminaIon Torrent
Paired endSingle end
Bacillus4 (86)2 (96)2 (95)
Clostridiaceae3 (92)1 (100)
Clostridium4 (80)2 (94)1 (100)
Corynebacterium2 (96)1 (100)1 (100)
Enterococcus3 (88)1 (100)1 (100)
Escherichia2 (96)1 (100)1 (100)
Lactococcus3 (88)3 (67)2 (99)
Pseudomonas3 (94)1 (100)2 (99)
Staphylococcus2 (90)2 (99)2 (99)
Streptococcus3 (90)2 (99)1 (100)
Other116 (12)111 (10)0
Sum14512713
  • a Results are based on the DADA2 analysis pipeline with the Greengenes database.

  • b Each value represents the average number of OTUs/ASVs (n = 3) and mean percentage of sequence reads assigned to the most abundant OTU/ASV within that taxon.