TABLE 3

OTU/ASV distribution of the 16S rRNA PCR amplicon mock community following Illumina MiSeq DNA sequencing without paired-end assembly

TaxonomyNo. (%) of OTUs/ASVs bya:
QIIME 1DADA2
GreengenesRDPGreengenesRDP
Bacillus9 (97)2 (99)3 (67)1 (100)
Clostridiaceae1 (100)1 (100)1 (100)
Clostridium3 (99)3 (99)3 (99)3 (99)
Corynebacterium9 (96)7 (95)2 (75)2 (75)
Enterococcaceae1 (100)
Enterococcus2 (99)2 (99)2 (60)2 (60)
Enterobacteriaceae4 (99)8 (93)
Escherichia/Shigella1 (100)
Escherichia1 (100)
Lactococcus6 (99)2 (99)1 (100)1 (100)
Pseudomonas11 (91)4 (99)2 (65)2 (65)
Staphylococcus12 (98)3 (99)2 (76)2 (76)
Streptococcus4 (92)2 (99)1 (100)1 (100)
Other6 (28)2 (91)22 (12)25 (89)
Sum68364040
  • a Each value represents the average number of OTUs/ASVs (n = 3) and mean percentage of sequence reads assigned to the most abundant OTU/ASV within that taxon.