TABLE 2

Strains and plasmids used in this study

Strain or plasmidDescriptionReference
S. meliloti strains
    Rm1021WT SU47; Smr92
    CL101Rm1021 ecfR1 corrected Smr22
    CL150Rm1021 ecfR1 pstC corrected Smr22
    CL309CL150 nifD::Tn5-233 Spr SmrThis study
    RFF702CL150 ΔrpoE1-ecfR1 SmrThis study
    RFF164CL150 ΔrpoE2-rsiA1 SmrThis study
    RFF716CL150 ΔrpoE3-ecfR3 SmrThis study
    RFF165CL150 ΔrpoE4-ecfR4 SmrThis study
    RFF272CL150 ΔrpoE5-SMb21687 SmrThis study
    RFF117CL150 ΔrpoE6-ecfR6 SmrThis study
    RFF344CL150 ΔrpoE7-ecfR7 SmrThis study
    RFF465CL150 ΔrpoE8-SMb20593 SmrThis study
    RFF343CL150 ΔrpoE9-SMb20029 SmrThis study
    RFF198CL150 ΔrpoE10-ecfR10 SmrThis study
    RFF300CL150 ΔfecI-fecR SmrThis study
    RFF118CL150 ΔrsiA2 SmrThis study
    RFF625cCL150 Δall-ECF σs/putative anti-σs SmrThis study
    RFF155CL150 ΔrpoH2This study
    RFF157CL150 ΔrpoH1This study
    RFF231CL150 ΔrpoH1 CL150 ΔrpoH2This study
    RFF299CL150 ΔrpoH1 CL150 ΔrpoE2This study
Plasmids
    pCAP11Broad-host-range expression
vector, melibiose inducible; Spr
76
    pF1087pCAP11 rpoE1 SprThis study
    pF1084pCAP11 rpoE2 Spr22
    pF1071pCAP11 rpoE3 SprThis study
    pF1085pCAP11 rpoE4 SprThis study
    pF1074pCAP11 rpoE5 SprThis study
    pF1088pCAP11 rpoE6 SprThis study
    pF1080pCAP11 rpoE7 SprThis study
    pF1086pCAP11 rpoE8 SprThis study
    pF1077pCAP11 rpoE9 SprThis study
    pCL139pCAP11 rpoE10 SprThis study
    pF1082pCAP11 fecI SprThis study
    pCL308pJQ200SK, to correct mdh mutation in RFF625; GmrThis study
    pF1323pJQ200SK, to make ΔrpoE1-ecfR1; GmrThis study
    pF1332pJQ200SK, to make ΔrpoE2-ecfR2; GmrThis study
    pF1322pJQ200SK, to make ΔrpoE3-ecfR3; GmrThis study
    pF1328pJQ200SK, to make ΔrpoE4-ecfR4; GmrThis study
    pF1340pJQ200SK, to make ΔrpoE5-SMb21687; GmrThis study
    pF1324pJQ200SK, to make ΔrpoE6-ecfR6; GmrThis study
    pF1343pJQ200SK, to make ΔrpoE7-ecfR7; GmrThis study
    pF1351pJQ200SK, to make ΔrpoE8-SMb20593; GmrThis study
    pF1342pJQ200SK, to make ΔrpoE9-SMb20029; GmrThis study
    pF1333pJQ200SK, to make ΔrpoE10-SMc01151; GmrThis study
    pF1341pJQ200SK, to make ΔfecI-fecR; GmrThis study
    pF1326pJQ200SK, to make ΔrpoH1; GmrThis study
    pF1327pJQ200SK, to make ΔrpoH2; GmrThis study
    pJQ200SKsacB vector; P15a ori; does not replicate in S. meliloti; Gmr85
    pRK600ColE1; provides RK2 transfer functions; Cmr93