TABLE 4

Biolog Phenotype MicroArray comparisons for WT strain CL150 compared to the all-ECF σ deletion strain RFF625ca

Plate, wellbSubstratecAUC of
CL150/AUC
of RFF625cd
P valueSubstrate descriptione
PM09, H06Sodium nitrate (100 mM)1.527.4E−08Osmolyte
PM15, D06Domiphen bromide no. 21.814.3E−07Quaternary ammonium
compound
PM19, D05Iodonitrotetrazolium
violet no. 1
1.447.4E−06Tetrazolium dye
PM02, C04d-Melezitose1.561.2E−05Carbon source, trisaccharide
PM20, H07Tolylfluanid no. 30.433.7E−05Antibacterial, phenylsulfamide
PM12, B12Polymyxin B no. 41.708.9E−05Antibacterial, cationic
peptide-fatty acid
PM18, F08Tinidazole no. 41.899.1E−05Antibacterial, nitroimidazole
PM19, C04Chlorhexidine no. 40.591.0E−04Disinfectant, cationic
bisbiguanide
PM12, E10Benzethonium chloride
no. 2
1.571.2E−04Quaternary ammonium
compound
PM04, E05O-Phosphoryl-ethanolamine0.583.3E−04Phosphorous source
PM11, H12Ofloxacin no. 41.484.1E−04Antibacterial, fluoroquinolone
PM13, B04Azlocillin no. 41.574.7E−04Antibacterial, penicillin
PM11, D02Capreomycin no. 21.691.3E−03Antibacterial, cyclic peptide
PM11, E08Enoxacin no. 41.952.1E−03Antibacterial, fluoroquinolone
PM09, E08Urea (3%)1.455.2E−03Osmolyte
PM13, G06Manganese(II) chloride
no. 2
1.305.5E−03Heavy metal
PM11, B12Lomefloxacin no. 41.406.2E−03Antibacterial, fluoroquinolone
PM11, C03Bleomycin no. 31.659.0E−03Antibacterial, peptide-
polyketide
PM19, A01Josamycin no. 11.269.6E−03Antibacterial, macrolide
PM04, A02Sodium phosphate1.351.0E−02Phosphorous source
PM20, B09Tetrazolium violet no. 11.402.4E−02Tetrazolium dye
PM04, H11Methane sulfonic acid0.764.2E−02Sulfur source
PM15, B06EDTA no. 21.794.6E−02Chelating agent
  • a Biolog Phenotype MicroArray comparisons were identified using the opm package (63 [see Materials and Methods]) for WT CL150 compared to the all-ECF σ deletion strain RFF625c.

  • b Shown are the plate number and then well number of Biolog Phenotype MicroArray 96-well plates. PM2, carbon sources; PM4, phosphorous and sulfur sources; PM9, osmolytes; PM11 to -20, chemical sensitivity tests for bacteria.

  • c For chemical stress tests, the number indicates which of the four concentrations (where 1 is lowest and 4 is highest) had a significant effect.

  • d AUC, area under the concentration-time curve (see Materials and Methods).

  • e Compounds listed as "antibacterial" possess antibacterial activity; however, their primary commercial use may not be treatment of bacterial infections (examples include tolylfluanid, tinidazole, and bleomycin).