TABLE 3

Phenotypic tests of RFF625c mutant, deleted for all ECF σ and putative anti-σ genes

TestaResult
Growth on LB agar platesSlightly slower than WT CL150
Growth on M9 sucrose agar platesIndistinguishable from WT CL150
Heat stress (37°C) on LB and M9Indistinguishable from WT CL150
Envelope stress (0.1% DOC)Indistinguishable from WT CL150
Envelope stress (3% or 10% SDS)Indistinguishable from WT CL150
Oxidative stress, exponential phase (1 mM H2O2)Indistinguishable from WT CL150
Oxidative stress, stationary phase (100 mM H2O2)Indistinguishable from WT CL150
Swim motilityIndistinguishable from WT CL150
EPS productionIndistinguishable from WT CL150
  • a Experimental details are described in Materials and Methods. DOC, sodium deoxycholate; SDS, sodium dodecyl sulfate.