TABLE 3 

Plasmids useda

PlasmidC. albicans
selection marker
E. coli selection
marker(s)
Parental
plasmid
ReferenceBCCM
accession no.
pC2HB-GCCmLEU2Kan, CmpC2HB32LMBP 9242
pC2HP-GCCdARG4Kan, CmpC2HP32LMBP 9244
pDONR207-XXGenpDONR20732
  • a XX denotes a specific ORF inserted in the Gateway donor vector pDONR207 from the C. albicans ORFeome project (32). For the creation of the C2H prey and bait library, ORFs were transferred from the pDONR207 plasmid to the pC2HB-GC or pC2HP-GC vectors using the Gateway LR reaction system according to the protocol in the Invitrogen manual.