TABLE 2 

SYBR green-based comparative qPCR of pol gene for PbAdV-1a

TissueCell lineCTfor DNA from cells
Passage 1 Passage 2 Passage 3
LiverCrFK3836.433
MDCK II
Vero
BHK
KidneyCrFK30
MDCK II29
Vero
BHK
Lymph nodeCrFK262831
MDCK II243028
Vero2931
BHK
  • a qPCR was performed on DNA extracted from cell culture of supernatant from liver, kidney, and lymph node tissue on the CrFK, MDCK II, Vero and BHK cell lines after 1, 2, and 3 passages. −, negative result.