TABLE 1 

Strains and plasmids used in this study

Strain or plasmidStrain genotype or plasmid constructionSource
Strains
    CNVY106α H99::GFP-tubulin-NAT (pLKB35)This study
    CNVY108α H99::GFP-H4-NAT (pVY3)35
    CNVY111a KN99::mCherry-CENP-A-NEO (pLKB74) GFP-NDC1-NAT (pVY4)35
    CNVY138a KN99::mCherry-CENP-A-NEO (pLKB74) SAD1::SAD1-GFP-NATThis study
    CNVY156α H99::CENP-Ap-GFP-CENP-A-NAT (pVY22)This study
    CNVY177α SPC98p::GAL7p-SPC98-HYG SPC98::SPC98-GFP-NAT H99::mCherry-CENP-A-NEO (pLKB74)This study
    CNVY182α H99 SAD1p::GAL7p-mCherry-SAD1-HYG GFP-NDC1-NAT (pVY4)This study
    CNVY191α SAD1::sad1-NEOThis study
    CNVY193a KN99::mCherry-CENP-A-HYG (pLKB75) GFP-NDC1-NAT (pVY4) SAD1::sad1-NEOThis study
    CNVY194α H99::GFP-tubulin-NAT (pLKB35) SAD1::sad1-NEOThis study
    CNVY200a KN99::CENP-Ap-GFP-CENP-A-NAT (pVY8) SAD1::sad1-NEOThis study
    CNVY210α H99::GFP-H4-NAT (pVY3) SAD1::sad1-NEOThis study
Plasmids
    pLKB35pCN19 + α-tubulin (BamHI-BamHI)35
    pLKB74pXLI + CENP-Ap-mCherry-CENP-A (NEO)35
    pLKB75pXLI + CENP-Ap-mCherry-CENP-A (HYG)35
    pVY1pCN19 + CENP-A (BamHI-BamHI)35
    pVY3pCN19 + H4 (BamHI-SpeI)35
    pVY4pCN19 + NDC1 (BamHI − SpeI)35
    pVY8GFP-CENP-A-NAT from pVY1 into pBSII KS using SacI-ApaIThis study
    pVY22CENP-Ap replaced H3p in pVY8This study