TABLE 7 

Strains used in this study

SpeciesIsolate or strainSourceDescriptionAntifungal testa
S. cerevisiaeW303 (ATCC 200060)Lab strain, obtained from Catherine Fox lableu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15CLSI M27-A3, 1 mM uracil
C. neoformansCN1Cerebrospinal fluid; clinical isolateAzole susceptibleCLSI M27-A3, tested by NIAID
CN2Popliteal lymph node; clinical isolate (animal)Fluconazole resistantCLSI M27-A3, tested by NIAID
CN3Cerebrospinal fluid; clinical isolateFluconazole resistantCLSI M27-A3, tested by NIAID
C. amylolentusCBS 6273Insect frass isolate, obtained from Joseph Heitman labCLSI M27-A3
CBS 6039Insect frass isolate, obtained from Joseph Heitman labCLSI M27-A3
C. albicansATCC 90028 (CA1)BloodCLSI M27-A3, tested by NIAID
CA2CLSI M27-A3, tested by NIAID
CA3BloodAzole resistantCLSI M27-A3, tested by NIAID
C. kruseiQCCLSI QC isolate for susceptibility testingCLSI M27-A3, tested by NIAID
C. aurisB11220Clinical isolate (Japan), obtained from David Andes labCLSI M27-A3
C54039Clinical isolate (Columbia), obtained from David Andes labAmphotericin B resistantCLSI M27-A3
Coccidioides sp.Cocci1Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
Cocci2Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
Cocci3Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
Cocci4Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
Cocci5Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
Cocci6Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
Cocci7Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
Cocci8Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
Cocci9Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
Cocci10Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
B. dermatitidisBD1Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
BD2Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
BD3Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
H. capsulatumHC1Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
HC2Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
HC3Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
HC4Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
HC5Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
HC6Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
HC7Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
HC8Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
HC9Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
HC10Unknown; NIAID preclinical testing servicesCLSI M38-A2, tested by NIAID
A. fumigatusCEA10Nancy Keller labCLSI M38-A2, 35°C
A. flavusNRRL3357Nancy Keller labCLSI M38-A2, 35°C
A. oryzaeRib40JGI Genome ProjectCLSI M38-A2, 35°C
A. terreusNCCB IH2624JGI Genome ProjectCLSI M38-A2, 35°C
A. parasiticusSu-1JGI Genome ProjectCLSI M38-A2, 35°C
N. fisheriCBS 544.65JGI Genome ProjectCLSI M38-A2, 35°C
A. nidulansFGSCA4JGI Genome ProjectCLSI M38-A2, 35°C
A. aculeatusCBS 172.66JGI Genome ProjectCLSI M38-A2, 35°C
A. carbonariusDT0115-B6JGI Genome ProjectCLSI M38-A2, 35°C
A. wentiiDT0136-E9JGI Genome ProjectCLSI M38-A2, 35°C
A. sydowiiCBS 593.65JGI Genome ProjectCLSI M38-A2, 35°C
A. foetidusCBS 106.47JGI Genome ProjectCLSI M38-A2, 35°C
A. zonatusCBS 506.65JGI Genome ProjectCLSI M38-A2, 35°C
A. nigerCBS 113.46JGI Genome ProjectCLSI M38-A2, 35°C
A. glaucusCBS 516.65JGI Genome ProjectCLSI M38-A2, 35°C
A. brasiliensisCBS 101740JGI Genome ProjectCLSI M38-A2, 35°C
A. clavatusCBS 513.65JGI Genome ProjectCLSI M38-A2, 35°C
A. versicolorCBS 795.97JGI Genome ProjectCLSI M38-A2, 35°C
P. expansumd1Apples from Israel in 2012CLSI M38-A2, 29°C
T. marneffeiFRR2161, CBS 334.59, ATCC 18224ATCCCLSI M38-A2, 29°C
P. variotiiQCCLSI QC isolateCLSI M38-A2, tested by NIAID
F. oxysporumFO1BloodClinical isolateCLSI M38-A2, tested by NIAID
FO2BoneClinical isolateCLSI M38-A2, tested by NIAID
FO3BloodClinical isolateCLSI M38-A2, tested by NIAID
S. apiospermumSA1ToeClinical isolateCLSI M38-A2, tested by NIAID
SA2Elbow tissueClinical isolateCLSI M38-A2, tested by NIAID
L. prolificansLP1Chest woundClinical isolateCLSI M38-A2, tested by NIAID
R. arrhizusRA1Nose tissueClinical isolateCLSI M38-A2, tested by NIAID
RA2Tissue upper extremityClinical isolateCLSI M38-A2, tested by NIAID
RA3Palate tissueClinical isolateCLSI M38-A2, tested by NIAID
F. depauperataCBS 7855Caterpillar isolate, obtained from Joseph Heitman labCLSI M38-A2, SD was used as the growth medium during antifungal testing
T. rubrumATCC 28188Alana Sterkel lab; UWb Department of Pathology and Laboratory MedicineClinical isolate; nailCLSI M38-A with modificationsc
M. canisUW10Karen Moriello lab; UW School of Veterinary MedicineWild-animal isolate; catCLSI M38-A with modificationsc
T. tonsuransCBS 112818Theodore White lab, Broad InstituteClinical isolate; cheekCLSI M38-A with modificationsc
P. destructansATCC MYA-4855Jeffrey Lorch lab; U.S. Geological SurveyWild-animal isolate; batCLSI M38-A with modificationsc
  • a The antifungal test and modifications are given. NIAID, National Institute of Allergy and Infectious Diseases.

  • b UW, University of Wisconsin.

  • c CLSI M38-A with modifications to test established hyphae rather than conidia for susceptibility. See the paragraph on the revised CLSI M38-A protocol for hyphae of filamentous fungi and dermatophytes in Materials and Methods.