TABLE 1

Guide availability in C. albicans, C. glabrata, N. castellii, and S. cerevisiaea

SpeciesNo. of
unique
guides
No. (%) of uniquely
targetable
genes
Avg no.
of unique
guides/gene
No. of
multigene
guides
No. (%) of
genes targetable
with other genes
C. albicans (old pipeline)550,0456,288 of 6,466 (97)87105,7476,023 of 6,466 (93)
C. albicans (new pipeline)333,4646,236 of 6,466 (96)5312,2204,647 of 6,466 (72)
C. albicans (allele specific, off-target cutoff of 0.2)13,2863,112 of 4,046 (77)4NANA
C. albicans (allele specific, off-target cutoff of 0.5)21,7653,464 of 4,046 (86)6NANA
C. albicans (allele specific, no off-target cutoff)39,2993,805 of 4,046 (94)10NANA
C. glabrata197,6045,285 of 5,597 (94)385,6992,554 of 5,597 (46)
N. castellii461,9325,566 of 5,594 (99)5613,2084,434 of 5,594 (79)
S. cerevisiae511,4396,398 of 6,600 (97)8014,6584,522 of 6,600 (69)
  • a NA, not applicable (allele-specific guides by definition do not target other genes); off-target cutoff score is calculated using software developed by Doench et al. (21). A guide targeting another place in the genome with an off-target cutoff higher than the indicated score is considered unspecific.