TABLE 1

Marginal association between variables of interest and overall microbial compositiona

VariableBray-Curtis
distance
Unweighted UniFrac
distance
Weighted UniFrac
distance
R2 (%)P valueR2 (%)P valueR2 (%)P value
gDNA concn (1 ng/μl to 1.28 × 10−5 ng/μl)31.0<0.00138.1<0.00122.4<0.001
16S rRNA gene yield42.8<0.00139.5<0.00120.7<0.001
Extraction method (Mo Bio vs Chemagic)0.80.570.7>0.790.60.78
PCR cycling method (standard vs touchdown)1.10.371.50.233.70.03
PCR volume (25 vs 50 μl)2.50.773.00.584.30.24
  • a Associations were assessed by using permutational multivariate analysis of variance, based on Bray-Curtis, unweighted UniFrac, and weighted UniFrac distances. Percentages of variance explained (R2) and P values are shown. Abbreviation: gDNA, genomic DNA.