TABLE 2

α-Diversitya

DNA reaction concn
(ng/μl)
No. of observed OTUs,
mean difference
(P value)b
Shannon index,
mean difference
(P value)c
Inverse Simpson index,
mean difference
(P value)d
2 × 10−1−0.664 (0.96)0.0138 (0.88)0.409 (0.78)
4 × 10−2−32.1 (0.06)−0.070 (0.43)−1.15 (0.41)
8 × 10−3−66.1 (<0.001)−0.167 (0.07)−3.22 (0.02)
1.6 × 10−3−131 (<0.001)−0.292 (0.002)−4.11 (0.004)
3.2 × 10−4−194 (<0.001)−0.450 (<0.001)−5.74 (<0.001)
6.4 × 10−5−233 (<0.001)−0.630 (<0.001)−6.23 (<0.001)
1.28 × 10−5−262 (<0.001)−0.926 (<0.001)−11.8 (<0.001)
  • a Comparison of each dilution with 1 ng/μl gDNA. Abbreviations: gDNA, genomic DNA; OTU, operational taxonomic units.

  • b The average number of observed OTUs was 362.

  • c The average Shannon index value was 4.14.

  • d The average inverse Simpson index value was 26.2.