TABLE 1

Strains and plasmids used in this study

Strain or plasmidGenotype or descriptionReference
or source
Strains
    P. aeruginosa PAO1
        MJ79Wild type14
        MJ80ΔgbdR15
        JM236ΔcdhRThis study
        JM253Wild type attTn7::88-89intYFPCFP-2This study
        JM339ΔcdhR attTn7::88-89intYFPCFP-2This study
        JM340ΔgbdR attTn7::88-89intYFPCFP-2This study
        MJ784ΔgbdR ΔcdhRThis study
    P. aeruginosa PA14
        MJ101Wild type42
        MJ11ΔcdhR11
        MJ26ΔgbdR15
        MJ262ΔcdhCA11
        JM179ΔcdhR ΔgbdRThis study
    E. coli
        MJ340Wild-type S17λpir
        DH5αNEB C2987NEB
        T7ExpressNEB C3016NEB
Plasmids
    pMQ30Suicide vector, Gmr36
    pMQ80High-copy-no. Pseudomonas vector, Gmr36
    pMal-C2XT7-expressing vector, MBP N-terminal tag, AmprNEB
    pTNS2Plasmid carrying attTn7 transposase43
    pUC18-mini-Tn7T-GmGmr on mini-Tn7T35
    pUC18-mini-Tn7T-Gm-eyfpGmr on mini-Tn7T with YFP35
    pUCP22High-copy-no. Pseudomonas stabilization vector, Gmr44
    pMW5lacZYA in pUCP2232
    pMW79PA14 genomic clone of PA5380-PA5389 in pMQ8011
    pPA5380KOgbdR deletion construct in pEX18-Gm15
    pJAM22Promoter caiX-lacZYA transcriptional fusion AThis study
    pJAM23Promoter caiX-lacZYA transcriptional fusion BThis study
    pJAM24Promoter caiX-lacZYA transcriptional fusion CThis study
    pJAM25Promoter caiX-lacZYA transcriptional fusion DThis study
    pJAM50PA5389 in pMal-C2XThis study
    pJAM76YFP-CFP in pMQ80This study
    pJAM86CFP PA5388-PA5389 intergenic region YFP in pUC18mini, DR2This study
    pJAM90PA5389 deletion construct in pMQ30This study
    pJAM122Promoter caiX-lacZYA transcriptional fusionThis study
    pJAM123Promoter caiX-lacZYA mut 1 transcriptional fusionThis study
    pJAM124Promoter caiX-lacZYA mut 2 transcriptional fusionThis study
    pJAM125Promoter caiX-lacZYA mut 3 transcriptional fusionThis study
    pJAM126Promoter caiX-lacZYA mut 4 transcriptional fusionThis study
    pJAM127Promoter caiX-lacZYA mut 5 transcriptional fusionThis study
    pJAM130Promoter caiX-lacZYA mut 6 transcriptional fusionThis study
    pJAM131C terminus of lacZ in pUCP22This study
    pJAM135PcdhRlacZYA in pUCP22This study