TABLE 1

Culture results for infected and noninfected TIVAP chambers from the two hospital cohorts and species (OTU) richness as detected by 16S clone library generationa

TIVAP group and
sample ID
Culture-isolated
organism(s)
No. of
CFU/ml
No. of OTUs detected
in 16S clone library
No. of clones generated
for 16S clone library
Curie symptomatic
    CS-1Sterile848
    CS-2 Staphylococcus aureus* >1,000148
    CS-3 Staphylococcus epidermidis* 200148
    CS-4 Staphylococcus epidermidis* >1,000148
    CS-5 Staphylococcus epidermidis* >1,000148
    CS-6 Candida parapsilosis >1,000548
    CS-7Sterile148
    CS-8 Staphylococcus aureus* 130645
    CS-9 Staphylococcus xylosus <10449
    CS-10 Candida krusei and Staphylococcus epidermidis* >1,000148
Curie asymptomatic
    CA-1Sterile948
    CA-2Sterile1048
    CA-3Sterile449
    CA-4Sterile449
    CA-5Sterile649
    CA-6Sterile448
    CA-7 Staphylococcus epidermidis 20348
    CA-8Sterile748
    CA-9Sterile953
    CA-10Sterile748
Limoges symptomatic
    LS-1 Staphylococcus epidermidis*,
Bacillus simplex
1, 1150
    LS-2Sterile647
    LS-3 Enterobacter cloacae* >500150
    LS-4(Staphylococcus epidermidis on
distal catheter)
248
    LS-5 Staphylococcus epidermidis* >500150
    LS-6 Escherichia coli* >500150
    LS-7 Staphylococcus aureus* <50149
    LS-8 Staphylococcus aureus* 1448
    LS-9 Pseudomonas aeruginosa* <50149
    LS-10 Staphylococcus epidermidis* <10249
Limoges asymptomatic
    LA-1Sterile752
    LA-2Sterile449
    LA-3Sterile548
    LA-4Sterile550
    LA-5Sterile348
    LA-6 Micrococcus luteus 1650
    LA-7 Micrococcus luteus 1350
    LA-8Sterile855
    LA-9Sterile448
    LA-10Sterile250
  • a Species shown in bold with an asterisk represent the cultured organism that was also the dominant organism detected in the 16S rRNA gene clone library. The bacteria isolated from the TIVAP samples of symptomatic patients were also isolated from the blood cultures.