Analysis of CRISPR-Cas9 efficiencies in wild-type and NHEJ mutant backgroundsa

StrainMean (SD)
No. of NATr colonies% ade2
RSY426 (ku70 lig4)531 (514)81 (17)
RSY376 (ku70)306 (200)49 (37)
RSY281 (parent haploid) 1,240 (1,144)25 (32)
  • a For this experiment, deletion constructs had long flanks (~1-kb homology flanking the ADE2 gene). n = 3 transformations per strain. The Cas9 and sgRNA are under a C. lusitaniae promoter.