TABLE 1

Sequences of ODNs used in this study

NameSequence
PML gRNA1 top5′CACCGCAATCTGCCGGTACACCGAC
PML gRNA1 bottom5′AAACGTCGGTGTACCGGCAGATTGC
PML gRNA2 top5′CACCGGGAACTCCTCCTCCGAAGCG
PML gRNA2 bottom5′AAACCGCTTCGGAGGAGGAGTTCCC
CAG gRNA top5′CACCGGTTCCGCGTTACATAACTTA
CAG gRNA bottom5′AAACTAAGTTATGTAACGCGGAACC
Surveyor gRNA1 fwd5′AATGGGGGTATTGGGGTGCTG
Surveyor gRNA1 rev5′TGGTCAGCGTAGGGGTGC
Surveyor gRNA2 fwd5′AAGAGTGGAATTTCTGGGTC
Surveyor gRNA2 rev5′GAAGCACTTGGCGCAGAGG
PML 5′arm fwd5′CTAGCGGCCGCATTTCATTTCTTTCTAAC
PML 5′arm rev5′AATTCTAGAGCCGCTGCAGACTCTC
PML 3′arm fwd5′TTACAATTGGGCTGTGTGCACCC
PML 3′arm rev5′CGCCCTGCAGGCTGTACGAATGTATTAC
MCS2 top5′CATGGCAATTGAAGCTTCCTGCAGGGGATCCA
MCS2 bottom5′CATGTGGATCCCCTGCAGGAAGCTTCAATTGC
Knock-in fwd5′TCTGGACGAAGAGCATCAGG
Knock-in rev5′GATTGCACTCTCTCTCTCCTC
WT/indel fwd5′ACACGCTGTGCTCAGGATGC
WT/indel rev5′GTTGCGCAGCTCTGCTAGG
Actin fwd5′CCTCCCTGGAGAAGAGCTA
Actin rev5′ACGTCACACTTCATGGA