TABLE 1

Comparison of C. albicans CRISPR systemsa

AttributeNguyen et al.Min et al./Huang and MitchellVyas et al.
Cloning-free gRNA assembly++
Markerless genome editing at target locus++
Recyclable markers+±b+
Edited strains do not retain CRISPR components++
Target-independent gRNA stitching protocol+NAc
  • a Comparison of key attributes of the three CRISPR systems that have been developed for use with C. albicans. Advantages (+) and limitations (−) are indicated.

  • b Marker excision in the Huang and Mitchell system is limited to subsequent transformations and requires the integration of a distinct marker at a second target locus.

  • c NA not applicable.