TABLE 1

Predicted cellulose and cello-oligosaccharide utilization genes in S. scabiesa

Category and geneFunctionCebR boxbPosition(s)
Cellulose, cello-oligosaccharide extracellular degradation
 scab1101 Secreted GH (probable β-glucosidase), GH5 familyND
 scab5981, celBCelB, secreted cellulase B (endoglucanase), GH12 family with CBM2 domain TGGGAGCGCTCCCA −82
 scab8871, cel1Secreted endoglucanase, GH9 family with CBM4 domain TGGGAGCGCTCCCA −90
 scab16431 Putative cellulase CelA1 (endoglucanase), GH6 family TGGGAGCGCTCCCA −113
TGGaAGCGCTtCCA −128
TGGGAGCGCTtCCg −264
 scab17001 Secreted cellulase (β-1,4-exocellulase), GH6 family with CBM2 domain TGGGAGCGCTCCCA −130
TGGaAcCGCTCCCA −283
 scab17011 Putative secreted cellulase, GH48 family with CBM2 domain TGGGAGCGCTCCCA −311
TGGaAcCGCTCCCA −158
 scab17021 Putative secreted cellulase, GH74 family with CBM2 domainNDc
 scab21081, celS2CelS2 secreted lytic polysaccharide monooxygenase with CBM2 domain (possible bldA regulation) TGGGAGCGCTCCCA −177
 scab51081 Secreted cellulase (beta-glucosidase?), GH5 family with CBM2 domain (possible bldA regulation) TGGGAGCGCTCCCA −54
 scab78881 Secreted GH, GH5 family with CBM2 and fn3 domains TGGGAGCGCTCCCA −85
 scab86271 Putative secreted hydrolase, GH5 familyND
 scab86311 Putative secreted endoglucanase, GH5 familyND
 scab86471 Putative endoglucanase (cellulase), GH5 family with CBM2ND
 scab89741 Secreted cellulose-binding protein with chitin (CBM3) binding domain TGGGAGCGCTCCCA −116
 scab90061 Putative cellulase, expansion, peptidoglycan-binding domain (CBM63) TGGGAGCGCTCCCA −201
 scab90081 Secreted cellulase B, GH12 family with CBM2 domain TGGGAGCGCTCCCA −60
 scab90091 Cellulose 1,4-β-cellobiosidase, GH48 family with CBM2 domain TGGGAGCGCTCCCA −105
TGGGAGCGgTtCCc −248
cGGGAGCGCTCCCA −674
 scab90101 Cellulose 1,4-β-cellobiosidase, GH6 family with CBM2 domain TGGGAGCGCTCCCA −761
TGGGAGCGgTCCCa −618
cGGGAGCGCTCCCA −192
Transport, intracellular degradation, and regulation
 scab57721, bglCBglC, putative intracellular β-glucosidase, GH1 family TGGaAGCGCTCCCA −13
 scab57751, cebECebE, cellobiose/cellotriose binding component of CebEFG-MsiK ABC-type transporter TGGGAGCGCTCCCA −130
 scab57761, cebRCebR, cellulose/cello-oligosaccharides utilization repressor, thaxtomin biosynthesis expression repressor TGGGAGCGCTCCCA −496
Phytotoxin biosynthesis
 scab31781, txtBTxtB, thaxtomin synthetase B gGGGAGCGCTCCCA +1507
 scab31791, txtATxtA, thaxtomin synthetase A cGGGAGCGCTCCCA −899
 scab31801, txtRTxtR, cellobiose-dependent thaxtomin biosynthesis transcriptional activator cGGGAGCGCTCCCA −786
  • a Abbreviations: CBM, cellulose-binding domain; fn, fibronectin; GH, glycosyl hydrolase; ND, not detected.

  • b Lowercase letters indicate nucleotides that do not match with the CebR consensus sequence TGGGAGCGCTCCCA.

  • c Probably regulated by the two CebR boxes identified upstream of the adjacent scab17011 gene.