TABLE 1

piggyBac mutants included in phenotypic screensa

piggyBac IDClosest gene IDGene descriptionStrandType of insertionDistance to gene (bp)ClonePCRQIseqWGS
PB-43PF3D7_0206100Cysteine desulfuration protein SufE+3′ UTR−293XXXX
PB-9PF3D7_0404600Conserved Plasmodium membrane protein, unknown function+Exon0XXX
PB-11PF3D7_0416500Repressor of RNA polymerase III transcription MAF1, putativeExon0XXX
PB-14PF3D7_0511500RNA pseudouridylate synthase, putative+Exon0XXXX
PB-17PF3D7_0521900Conserved Plasmodium protein, unknown function+Exon0XXXX
PB-3PF3D7_0615900Conserved Plasmodium protein, unknown functionExon0XXXX
PB-125PF3D7_0622900Transcription factor with AP2 domain(s), putative (ApiAP2)Intergenic−2,407XXX
PB-20PF3D7_0808700Erythrocyte membrane protein 1, PfEMP1 (VAR)+Intron0XXX
PB-1PF3D7_0811300CCR4-associated factor 1 (CAF1)+Exon0XXXX
PB-54PF3D7_0902200Serine/threonine protein kinase, FIKK family (FIKK9.3)Exon0XXX
PB-22PF3D7_0931000Elongation factor Tu, putativeExon0XXX
PB-12PF3D7_1018300Conserved Plasmodium protein, unknown functionIntergenic−834XXX
PB-25PF3D7_1035800Probable protein, unknown function (M712)+Exon0XXXX
PB-4PF3D7_1122900Dynein heavy chain, putative+Exon0XXX
PB-19PF3D7_1133700Conserved Plasmodium protein, unknown function+Exon0XXXX
PB-21PF3D7_1136000Conserved Plasmodium protein, unknown function+Exon0XXXX
PB-28PF3D7_1138900ncRNA/unspecified product+Exon0XXX
PB-5PF3D7_1141900Inner membrane complex protein 1b, putative (IMC1b)Exon0XXX
PB-33PF3D7_1207800Conserved Plasmodium protein, unknown functionExon0XXXX
PB-18PF3D7_1219300Erythrocyte membrane protein 1, PfEMP1 (VAR)Exon0XXX
PB-24PF3D7_1231800Asparagine-rich protein, putative+Exon0XXX
PB-2PF3D7_1305500MAPK phosphatase 1, putative (MKP1)+Exon0XXXX
PB-58PF3D7_1343700Kelch protein K13Intergenic−1,034XXX
PB-120PF3D7_1459500Conserved Plasmodium protein, unknown function+5′ UTR+244XXX
PB-6PF3D7_1475700Tubulin epsilon chain, putative+Intergenic+908XXX
  • a Insertion positions were determined by QISeq. Positive distances indicate insertions upstream of the translation start site. Negative distances indicate insertions downstream of the translation stop site. Validation of mutants included limiting-dilution cloning, TAIL PCR and QIseq to verify the transposon insertion site, and WGS of select mutants to confirm that there were no other major genomic changes.