TABLE 2

Plasmids used in this study

PlasmidRelevant marker(s)Reference
YCplac33 URA3 CEN-ARS 34
pRS316-GAL-LRG1 URA3 CEN-ARS pGAL-LRG1-LRG1 3′ UTRThis study
YCplac33-ROM2myc URA3 CEN-ARS pROM2-ROM2myc-ADH1 3′ UTRThis study
pRS316-GAL-HA-ROM2 URA3 CEN-ARS pGAL-HA-ROM2-ROM2 3′ UTRThis study
YCplac33-RHO1-Q86L URA3 CEN-ARS RHO1-Q86L 35
pRS316-PKC1-R398P URA3 CEN-ARS PKC1-R398P 36
YEplac195 URA3 34
YEplac195-POP2 URA3POP2 7
YEplac195-DHH1 URA3DHH1 7
YEplac195-CCR4 URA3CCR4 7
YEplac195-ROM2 URA3ROM2 11
pCgLEU2 C. glabrata LEU2 in pUC19 27
pCgHIS3 C. glabrata HIS3 in pUC19 27
pCgTRP1 C. glabrata TRP1 in pUC19 27
pKlURA3 K. lactis URA3 in pUC19 11
pKlURA3 K. lactis URA3 in pUC19 11
pFA6a-13myc-kanMX6myc 30