TABLE 2

Escherichia coli and Vibrio cholerae strains used in this studya

StrainDescriptionReference or source
Escherichia coli
S17-λpirrecA thi pro hsdR M+ [RP4-2-Tc::Mu::Kmr Tn7] (λpir) Tmpr Strrde Lorenzo and Timmis (49)
Vibrio cholerae
    O395Classical wild type, StrrSon et al. (9)
    C6706El Tor wild type, StrrSon et al. (9)
    N16961El Tor reference strain, StrrSon et al. (9)
    MQ1795El Tor variant, clinical isolateNair et al. (27)
    MS0005Classical O395ΔtoxT, StrSon et al. (9)
    MS0014Classical O395ΔtcpA, StrKirn et al. (50)
    MS0235El Tor N16961 hapR* StrThis study
    MS0245El Tor N16961 hns* StrThis study
    MS0230El Tor N16961 luxO* StrThis study
    MS0240El Tor N16961 vieA* StrThis study
    MS0256El Tor N16961 hapR* hns* StrThis study
    MS0254El Tor N16961 hapR* luxO* StrThis study
    MS0251El Tor N16961 hapR* vieA* StrThis study
    MS0285El Tor N16961 hns* luxO* StrThis study
    MS0281El Tor N16961 hns* vieA* StrThis study
    MS0287El Tor N16961 luxO* vieA* StrThis study
    MS0279El Tor N16961 hapR* hns* luxO* StrThis study
    MS0295El Tor N16961 hapR* hns* vieA* StrThis study
    MS0297El Tor N16961 hapR* luxO* vieA* StrThis study
    MS0299El Tor N16961 hns* luxO* vieA* StrThis study
    MS0282El Tor N16961 hapR* hns* luxO* vieA* StrThis study
  • a S17-λpir was used to introduce all possible SNPs and SNP combinations into the El Tor reference strain N16961, using a previously described allelic-exchange protocol (46). SNPs introduced are indicated by asterisks. Tmpr represents trimethoprim resistance, and Strr represents streptomycin resistance.