TABLE 2

Recovery of unselected markers among Ade transformantsa

ExperimentTransformant
selection
No. of red transformants by phenotypebTotal no. of
transformants
NatSNatRArgArg+
1Arg+173NA2020
2Arg+158NA2323
3Arg+2615NA4141
3NatRNA2015520
  • a ade2Δ::ARG4 and ade2Δ::NAT deletion templates were mixed in a 1:1 molar ratio. BWP17 cells were transformed with the mixture along with the CaCAS9 and sgRNA ADE2.1 PCR products. Transformants were selected through either an Arg+ or NatR phenotype, as indicated in the table. Red transformants were purified and scored for the unselected marker. The results of three independent experiments are shown.

  • b NA, not applicable because the selection did not allow recovery of the phenotype.