TABLE 1

Transformation frequencies with the transient CRISPR systema

CaCAS9
expression
cassette
sgRNA
expression
cassette
ade2Δ::NAT
deletion
construct
NAT
resistance
frequency
Homozygous
ADE2 deletion
frequency
+1.4 × 10−60.0
++8.3 × 10−70.0
ADE2.1 +8.3 × 10−70.0
+ ADE2.1 +4.7 × 10−52.1 × 10−5
+ ADE2.1 0.00.0
ADE2.2 +1.3 × 10−60.0
+ ADE2.2 +2.1 × 10−51.4 × 10−5
+ ADE2.2 0.00.0
0.00.0
  • a Transformation frequency was calculated as the ratio of the number of colonies on YPD+NAT medium divided by the number on nonselective YPD medium. Each transformation experiment was repeated four times.