TABLE 1

Metadata for B. pertussis isolates collected during the California 2010 and Vermont 2012 statewide epidemics and vaccine strains that were selected for whole-genome sequencingl

IsolateLocationYr of
isolation
Age at
symptom
onset
Vaccination
status
PFGE
type
MLVAaprnbptxPbptxAbfimHb,cprn
genotype
Prn
productiond
H374CA2010InfantUVCDC002162311WT prn+
H375eCA2010InfantUVCDC2681861121Del nt 26-109f
H378CA2010InfantUVCDC253272311IS481::240 Fwdg
H379CA2010InfantUVCDC046272312WT prn+
H380eCA2010InfantUVCDC013272312WT prn+
H489CA2010InfantUVCDC082272312WT prn+
H542eCA2010InfantUVCDC269272311WT prn+
H559eCA2010InfantUVCDC253272311IS481::240 Fwdg
H561CA2010InfantUVCDC170162312WT prn+
H563eCA2010Infant≥1 doseCDC271272312WT prn+
H564CA2010ChildUVCDC013272312WT prn+
H622CA2010InfantUVCDC217272311WT prn+
H627CA2010InfantUVCDC217272311WT prn+
H788VT2011InfantUVCDC0461282312WT prn+
I669VT2011AdultUVCDC013272312WT prn+
I468VT2012ChildUVCDC002272311SC @ nt 1273
I469VT2012Child≥1 doseCDC342272312IS481::1613 Revh
I472VT2012AdolescentUVCDC046272312IS481::1613 Revh
I475VT2012AdultUVCDC237272311IS481::1613 Fwdi
I476VT2012AdolescentUTDCDC300272311IS481::1613 Fwdi
I480VT2012Child≥1 doseCDC217272311WT prn+
I483VT2012InfantUTDCDC237272311IS481::1613 Fwdi
I488VT2012Child≥1 doseCDC343272311IS481::1613 Fwdi
I496VT2012ChildUTDCDC343272311IS481::1613 Fwdi
I498VT2012Adult≥1 doseCDC253272311IS481::240 Revj
I517VT2012Child≥1 doseCDC344272311IS481::1613 Fwdi
I518VT2012ChildUTDCDC002272311SC @ nt 1273
I521VT2012Child≥1 doseCDC237272311IS481::1613 Fwdi
I538VT2012ChildUNKCDC002272311prnP (–74 nt)k
I539VT2012ChildUTDCDC002272311prnP (–74 nt)k
I646VT2012ChildUVCDC274272311WT prn+
I656VT2012ChildUTDCDC010272311WT prn+
I707VT2012ChildUTDCDC253272311WT prn+
C393China1951UNKNACDC052UNK1121WT prn+
E476Japan1954UNKNACDC232381121WT prn+
  • a MLVA type is defined by the repeat counts for VNTR1, VNTR3, VNTR4, VNTR5, and VNTR6 (21).

  • b Single-copy locus (21).

  • c Formerly referred to as fim3.

  • d Based on enzyme-linked immunosorbent assay.

  • e Fatal cases.

  • f Signal sequence deletion (nt 26 to 109).

  • g IS481 forward insertion at nt 240.

  • h IS481 reverse insertion at nt 1613.

  • i IS481 forward insertion at nt 1613.

  • j IS481 reverse insertion at nt 240.

  • k Promoter disruption (–74 nt), previously identified as promoter inversion (11, 15).

  • l Abbreviations: MLVA, multilocus variable-number tandem-repeat analysis; CA, California; VT, Vermont; nt, nucleotide; UTD, up to date; UV, unvaccinated; UNK, unknown; WT, wild-type; SC, stop codon; NA, not applicable.