TABLE 3

Plasmids used in the study

PlasmidDescriptionReference or source
pGTC101Source of chloramphenicol cassette67
p11086Source of kanamycin cassette in D. radioduransLaboratory stock
pET21dPET expression system, pT7lac, C-terminal 6His tag, AmprNovagen
pET26bPET expression system, pT7lac, C-terminal 6His tag, KanrNovagen
pET21dPET expression system, pT7lac, N-terminal 6His tag, KanrNovagen
pET21d-pprApET21d NcoI/XhoI + PCR fragment containing pprAThis work
pET26b-gyrApET26b NdeI/XhoI + PCR fragment containing gyrAThis work
pET30EK/LIC-gyrBpET30Ek/LIC + PCR fragment containing gyrBThis work