TABLE 4

Plasmids used in this study

PlasmidVectorDescriptionResistanceaReference or source
pEF6-hTLR5pEF6-V5Protein expression vector for human TLR5Ampr 86
pEF6-V5Mammalian protein expression vector, originated from pEF6-TLR5-V5 by
excision of the insert, EF-1α promoter, V5 tag
Ampr 13
pCJ801pEF6-V5Protein expression vector for chicken TLR5 (pEF6-chTLR5-V5)AmprE. Faber and C. Josenhans,
unpublished data
pET28aProtein expression vector, T7 lac promoter, 6×His tag, T7 tagKmrNovagen, Darmstadt,
Germany
pCJ375pET28a S. enterica serovar Typhimurium LT2 fliC cloned into pET28a via BamHI and XhoIKmrS. K. Lee and C. Josenhans,
unpublished data
pCJ1024pET28a flaC (C. jejuni, strain NCTC 11351) cloned into pET28a via BamHI and XhoIKmrThis study
pCJ1025pUC18 flaC::Cm (C. jejuni strain 11168)Ampr CmrThis study
pCJ1405pUC18 rdxA::Gm (C. jejuni strain 81-176) + flaC (C. jejuni strain 11168)Ampr GmrThis study
  • a Ampr, ampicillin resistance; Kmr, kanamycin resistance; Cmr, chloramphenicol resistance; Gmr, gentamicin resistance.