TABLE 2

Designation of group-specific OTU significantly different between microbiotas of FlaC-treated and mock-treated chickens

Specificity and OTUaClass or orderFamilyGenus (cluster)Most closely related sequence
(% nucleotide identity)
Mock-treated group
    OTU 63 Erysipelotrichales Erysipelotrichaceae Erysipelatoclostridium Clostridium innocuum (93)
    OTU 73 Lactobacillales Lactobacillaceae Lactobacillus Lactobacillus ingluviei (98)
    OTU 87 Clostridiales Clostridiaceae Clostridium (cluster IV) Clostridium sp. strain YIT12069 (100)
    OTU 134 Clostridiales Oscillospiraceae Oscillibacter ND
FlaC-treated group
    OTU 50 Erysipelotrichales Erysipelotrichaceae Clostridium (cluster XVIII)ND
    OTU 52 Clostridiales Lachnospiraceae Blautia Blautia faecis (98)
    OTU 121 Clostridiales Lachnospiraceae Blautia Blautia hydrogenotrophica (97)
  • a Designation of group-specific OTU at the genus or species level was verified by nucleotide BLAST search of 16S rRNA gene amplicon sequences using the NCBI nr database or SINA alignment with sequences listed in the Silva, Living Tree Project (LTP), or Ribosomal Database Project (RDP) database (http://www.arb-silva.de ). Noted identity scores (percentages) reflect similarities of OTU-specific consensus nucleotide sequences with the identified closest bacterial relatives. ND, not determined due to low similarity (<90%) of database hits.