TABLE 3

Protein expression changes in 48 h D. acidovorans WT15 and MT51 biofilms following chlorhexidine treatment at 0, 10 (W10 and M10), and 30 (W30 and M30) µg/ml after 24 h of biofilm growth

Biological process and spot no.ProteinAccession no.MasspIFold changeb2-way ANOVAc
MC/WCW10W30M10M30M10/W10M30/W30
Generation of precursor metabolites and energy (GO:0006091)a
115ATP synthase subunit alphagi|16089586755,2055.64+4.07+2.76+2.91NSNSNS+2.16S, T, I
122ATP synthase subunit alphagi|16089586755,2055.64+2.81+1.90+2.45NS+1.81+2.07+2.07S, T, I
125ATP synthase subunit betagi|16089586950,6335.23+2.68+2.22+3.08NS+2.84+1.98+2.48S, T, I
995ATP synthase subunit betagi|16089586950,6335.23+2.01+2.12+2.24+1.88+1.75+1.79NSS, T
589Ubiquinol-cytochrome c reductase iron-sulfur subunitgi|16090093521,6956.1NS−1.84−2.80NSNSNSNST, I
200Enolasegi|16090054045,9514.82+2.24+2.92+3.27+2.98+3.54+2.29+2.43S, T
Fatty acid metabolic process (GO:0006631)
478Malonyl CoA-acyl carrier protein transacylasegi|16090070334,3685.32+2.21+1.35+1.82NSNSNSNSS, T
770(3R)-Hydroxymyristoyl-ACP dehydratasegi|16090036916,5795.92NSNSNS+1.72+2.22NSNSS, T, I
563Enoyl-CoA hydratase/isomerasegi|16089697627,9945.39+2.50NSNS−2.63−3.82NSNST, I
371Basic membrane lipoproteingi|16089758541,0685.71NS+3.30+6.33+7.62+6.20NSNSS, T, I
Nucleotide biosynthetic process (GO:0009165)
606Glutamine amidotransferasegi|16089915025,0225.72−15.19−3.63−3.42NSNS−5.09−3.46S, T, I
218Amidohydrolasegi|16089702945,9405.03NS+2.11+2.43+1.92+2.16NSNSS, T
91Bifunctional phosphoribosylaminoimidazole-carboxamide formyltransferase/IMP cyclohydrolasegi|16089701157,1895.72+2.26+1.79+2.18NSNSNSNSS, T
Molecular chaperones / protein folding (GO:0006457)
877FKBP-type peptidylprolyl isomerasegi|16090049212,3145.23−2.02−1.73−1.53NSNS−1.59−1.83S, T
544PpiC-type peptidyl-prolyl cis-trans isomerasegi|16089873928,9148.5−1.69NSNS−1.50−2.33−1.93−2.58S, T
886Heat shock protein HSP20gi|16089782213,6085.78−9.43−7.54−7.49NSNSS, T, I
5960-kDa chaperoningi|16090109257,1235.02NS+1.85+3.36+3.35+ 7.0+1.59+1.84S, T, I
8560-kDa chaperoningi|16090109257,1235.02NSNSNSNS+2.92+2.05+2.14S, T, I
8660-kDa chaperoningi|16090109257,1235.02NSNSNS+2.03+2.49+1.77+1.89S, T, I
Translation/transcription (GO:0006412/0006351)
95Aspartyl/glutamyl-tRNA amidotransferase subunit Agi|16089580152,7275.51+2.30+2.47+2.65+2.19+2.35+2.04NSS, T
190Elongation factor Tugi|16089583843,2965.48NS+2.06+2.38+2.53+3.25NS+2.42S, T, I
274Elongation factor Tugi|16089583843,2965.48NS+3.81+4.31NS+2.01NSNST, I
436Elongation factor Tsgi|16090037931,1425.5NSNS+2.07+1.76+2.06NSNST
629Two-component LuxR family transcriptional regulatorgi|16089941623,8965.87NS−1.64−1.97NSNSNSNSS, T, I
Electron carrier activity (GO:0009055)
292Taurine catabolism dioxygenase TauD/TfdAgi|16090018937,3955.17NS+2.17+2.49NSNS−1.83NSS, T, I
612Electron transfer flavoprotein subunit alpha/betagi|16089684926,7347.64NSNS+1.66+1.96+3.08+1.57+1.67
Oxygen and reactive oxygen species metabolic process (GO:0006800)
662Superoxide dismutasegi|16089901121,6145.87NS−3.76−4.56NSNSNS+3.39S, T, I
647Superoxide dismutasegi|16089811227975.86−2.19NSNSNSNSNS−3.62
644Hypothetical protein (alkylhydroperoxidase-like protein, AhpD family)gi|16089971923,9675.87NSNSNS−3.03NS−2.92NSS, T, I
Proteolysis (GO:0006508)
658ATP-dependent Clp protease proteolytic subunitgi|16089808622,2745.45NSNS+1.49+1.77+1.87NSNSS, T, I
75Hypothetical protein (peptidase dimerization domain protein)gi|16089665662,7437.08NSNSNS−2.66−2.48−2.58−2.51S, T, I
Antibiotic metabolic process (GO:0016999)
536Hydroxyacylglutathione hydrolasegi|16090032928,0665.34+6.28NSNS−2.59NS+3.77+4.23S, T
Phosphorus metabolic process (GO:0006793)
687Inorganic diphosphatasegi|16090031119,3684.96NSNSNS+1.43+2.02NSNST, I
Cell redox homeostasis
734Redoxin domain-containing proteingi|16089585020,3835.63NSNSNSNS−2.78NSNSS, T, I
Transaminase activity (GO:0008483)
319Class V aminotransferasegi|16089652241,5746.08NSNSNS−1.96−6.12−2.05−4.33S, T, I
Ion bonding (GO:0043167)
416Hypothetical protein (ferritin-like superfamily of di-iron-containing four-helix-bundle protein)gi|16090129634,3434.86+3.97NSNS−2.45−2.71+2.23+2.26S, T
Others/unknown
595Glutathione S-transferase domain-containing proteingi|16089630023,0886.17NSNSNSNS+2.41+1.73NSS, T, I
808Ribosomal subunit interface proteingi|16089636713,6105.51−1.96NSNSNS−1.96−1.87−2.64S, T
46AMP-binding domain-containing proteingi|16090140663,8175.63NSNS−1.62−4.47−7.14−3.13−2.98S, T, I
714Phasin family proteingi|16090017419,1105.43−2.35−2.14−2.11NSNSNSNSS, T, I
  • a GO, gene ontology ID.

  • b Fold change in protein expression from the respective control biofilms. Minus and plus signs indicate decreased and increased expression. NS, not significant.

  • c S, strain (WT15 and MT51); T, treatment (0, 10, and 30 μg ml−1); I, interaction.