↵a FRAP parameter data include mean percentages of immobile fractions and times of equilibrium and their standard deviations for mScarlet and cytosolic GFP across all bleaching experiments. Cytosolic GFP (LDH2-GFP) is not expressed at pH 7. The Welch two-sample

*t*test was used to compare the mean immobile fractions or the times of equilibrium between the mScarlet and GFP fluorophores (*n*= 21 for each experiment).