TABLE 2

PCR primers used in this study

Gene targetForward primer(s)
(5′–3′)
Reverse primer(s)
(5′–3′)
Component(s)
surveyed
Sample
site(s)
Reference(s)
Bacterial 16S rRNA gene
V6 region
CTAACCGANGAACCTYACC,
CNACGCGAAGAACCTTANC,
CAACGCGMARAACCTTACC,
ATACGCGARGAACCTTACC
CGACRRCCATGCANCACCTAllUWM RAS72
Bacterial 16S rRNA gene
V4-V5 region
CCAGCAGCYGCGGTAANCCGTCAATTCNTTTRAGT,
CCGTCAATTTCTTTGAGT,
CCGTCTATTCCTTTGANT
AllAll73
Archaeal 16S rRNA gene
V4-V5 region
GCCTAAAGCATCCGTAGC,
GCCTAAARCGTYCGTAGC,
GTCTAAAGGGTCYGTAGC,
GCTTAAAGNGTYCGTAGC,
GTCTAAARCGYYCGTAGC
CCGGCGTTGANTCCAATTBiofiltersAll biofilter samples74
Betaproteobacterial amoAGGGGHTTYTACTGGTGGTCCCCTCKGSAAAGCCTTCTTCBiofiltersAll Biofilter Samples75, 76
Comammox amoAGGAYTTYTGGNTNGATTGGAWRKTNNGACCACCASKACCABiofiltersAll biofilter samplesModified from
reference 58
Nitrospira nxrBTACATGTGGTGGAACACGGTTCTGGTCRATCABiofiltersAll biofilter samples53