TABLE 2

Primers

PrimerSequence (5′–3′)a
CaDAL81-KpnI-FwGAAggtaccGAGAGAGCGGCAATGAAATC
CaDAL81-BamHI-RvGATCggatccTCATTTGGTTAGTTCGGGTG
CaDAL81-comp5V-FwAGTGGTTGCCGTTTTTTTTC
CaDAL81-comp5V-RvTGGGTAGTGGGGATATTTGG
CdHIS1-comp3V-FwGATCCACTAGTTCTAGAGCG
CdHIS1-comp3V-RvGGGTGTTGCCGACGCCATTG
  • a Restriction sites are indicated in lowercase letters.