TABLE 4

All 16 R. mucilaginosa strains bound in the anti-Rhodotorula capsule antibody assay, as well as in the WGA, CFW, and ConA assays, but did not bind EY tightlya

ARY strainIntensity of fluorescence
Rh1WGACFWConAEY
Rhodotorula, environmental
    115++++++++++++±
    116+++++++++++
    120+++++++++++
    126+++++++++++±
    162++++++++++++
    200+++++++++++
    225+++++++++++++
    250++++++++++++
Rhodotorula, clinical
    201+++++++++++
    202++++++++++++
    203+++++++++++
    204+++++++++++
    205+++++++++++±
    206++++++++++++±
    207+++++++++++±
    208+++++++++++±
Cryptococcus, clinical
    101 (H99)+++++++++++
    177 (NIH 117)++++++++++
  • a R. mucilaginosa and C. neoformans strains were grown in YPD media overnight at 30°C, and equivalent cell amounts were incubated with the anti-Rhodotorula capsule antibody, Rh1, followed by the FITC-conjugated goat anti-rabbit IgG secondary antibodies as described in Materials and Methods and shown in Fig. 5. Surface probes of FITC-WGA, CFW, EY, or FITC-ConA were incubated as described in Materials and Methods and shown in Fig. 6. The intensity of fluorescence was scored, in a manner similar to what was reported previously for C. neoformans (42), as follows: ++++, intense fluorescence (generated for C. neoformans with CFW after viewing these cells); +++, bright fluorescence; ++, moderate fluorescence; +, low fluorescence; ±, thin rim of fluorescence (seen after viewing EY-tagged cells); −, no fluorescence. Cells were randomized before being viewed, and sample sets were viewed consistently by one individual.