All 16 R. mucilaginosa strains bound in the anti-Rhodotorula capsule antibody assay, as well as in the WGA, CFW, and ConA assays, but did not bind EY tightlya

ARY strainIntensity of fluorescence
Rhodotorula, environmental
Rhodotorula, clinical
Cryptococcus, clinical
    101 (H99)+++++++++++
    177 (NIH 117)++++++++++
  • a R. mucilaginosa and C. neoformans strains were grown in YPD media overnight at 30°C, and equivalent cell amounts were incubated with the anti-Rhodotorula capsule antibody, Rh1, followed by the FITC-conjugated goat anti-rabbit IgG secondary antibodies as described in Materials and Methods and shown in Fig. 5. Surface probes of FITC-WGA, CFW, EY, or FITC-ConA were incubated as described in Materials and Methods and shown in Fig. 6. The intensity of fluorescence was scored, in a manner similar to what was reported previously for C. neoformans (42), as follows: ++++, intense fluorescence (generated for C. neoformans with CFW after viewing these cells); +++, bright fluorescence; ++, moderate fluorescence; +, low fluorescence; ±, thin rim of fluorescence (seen after viewing EY-tagged cells); −, no fluorescence. Cells were randomized before being viewed, and sample sets were viewed consistently by one individual.