TABLE 2

Genetic characteristics and phenotypic antimicrobial resistance patterns of multiresistant E. coli isolates and transconjugantsa

Isolate IDOriginMLST result withb:Isolate AMR profilecAMR genesPlasmid(s)pMLSTTransconjugant AMR profiledReplicon(s)g
7 genes8 genes
47ChildST517TE-G-SXT-S-AM-CFblaTEM-1B, dfrA8, qnrB19, strA, strB, tetBIncFII(pRSB107), IncFIB(AP001918)FII43, FIB11TE-G-SXT-S-AM-CFL, P, X3, FIIS, FIC, FII
52ChildST349TE-G-SXT-S-AM-CFblaTEM-1B, dfrA7, dfrA8, strA, strB, sul1, sul2, tetAIncFII(pHN7A8), IncFII, IncQ1FI33, FIB29TE-G-SXT-S-AM-CFP, I1γ, FIIS, FIC, FII
145ChildST4577TE-G-SXT-S-AM-CFblaTEM-1B, dfrA8, strA, strB, sul2, tetBIncFIB(pLF82), IncFII(pHN7A8)FII11TE-G-SXT-S-AM-CFFIA, W, A/C, FIIS, X2, FII
157ChildST226ST681TE-G-SXT-S-AM-CFaadA1, blaTEM-1B, dfrA15, qnrB19, tetAIncFII(pSE11), IncFIB(AP001918), IncFII, Col(MG828)FI79, FIB28TE-G-SXT-S-AM-CFP, A/C, FIIS, X2, FII
159ChildST226ST681TE-G-SXT-S-AM-CF-C-CTX-AMC*aadA1, blaTEM-1B, dfrA15, qnrB19, sul1, tetAIncFII(pSE11), IncFIB(AP001918), IncFII, Col(MG828)FI79, FIB28TE-G-SXT-S-AM-CF-C-CTX-AMCeP, A/C, FIIS, X2, FII
TE-S-AM-AMCeA/C, FIIS, X2, FII
211ChickenST8061ST305TE-G-SXT-S-AM-CF-C-CTX-AMC-CIP*aadA1, blaCMY-2, blaTEM-1B, catA1, cmlA1, floR, fosA, lnuF, qnrB19, strA, strB, sul2, sul3, tetAIncB/O/K/Z, IncFII, IncFIB(AP001918), IncQ1FII64, FIB27FIB, L, P, FIIS, FIIh
TE-G-SXT-SfFIB, FIIS, FII
191PigST8061ST305TE-G-SXT-S-AM-CF-C-CTX-AMC-CIP*blaCMY-2, blaTEM-1B, catA1, qnrB19, strA, strB, sul2, tetAIncFIB(AP001918), IncFII, IncB/O/K/Z, IncQ1FII64, FIB27TE-G-SXT-S-AM-CF-C-CTX-AMC-CIPFIB, L, P, A/C, FIIS, FII
58ChickenST189STNEW4TE-G-SXT-AM-C-CIP†dfrA14, strA, strB, sul2IncI2, IncYFI43Not conjugatedI2, L, A/C, FIIS, FIIi
132ChickenST48TE-G-SXT-AM-C†aadA1, aadA2, blaTEM-1B, cmlA1, dfrA12, dfrA14, mef, qnrB19, strA, strB, sul2, sul3, tetA, tetBIncFII(29), IncFIB(K), IncFIA(HI1)FII29, FIA13TE-G-SXT-AM-CL
19DogST101TE-G-SXT-S-AMaadA2, blaTEM-1B, dfrA12, sul3, tetA, tetMIncFII, IncN3FII34TE-G-SXT-S-AML, FIIS, Y, FII
44CatST10ST2TE-G-SXT-S-AMblaTEM-1B, dfrA8, strA, strB, sul2, tetBIncFIB(AP001918), IncFII(pRSB107)FII1, FIB54TE-G-SXT-S-AML, FIIS, Y, FII
90PigSTNEW1TE-G-SXT-S-AMblaTEM-1B, dfrA8, strA, strB, sul2, tetBIncFII(pRSB107), IncFIB(AP001918), IncI1FII48, FIB25TE-G-SXT-S-AMeL, FIIS, Y, FII
TE-G-S-AMeI2, FIB, L, P, FIIS, Y, FII
113ChildST226ST681TE-G-SXT-S-AMblaTEM-1B, dfrA8, strA, strB, sul2, tetAIncFIB(AP001918), IncFII(pRSB107), IncFII(29)FII1, FIB54TE-G-SXT-S-AMeI2, FIB, P, FIIS, Y, FII
TE-G-S-AMeI2, FIIS, FII
169ChildSTNEW2TE-G-SXT-S-AMblaTEM-1B, dfrA8, strA, strB, sul2, tetBIncFIB(AP001918), IncFII, IncFII(pRSB107)FII1, FIB54TE-G-SXT-S-AMFIIS, FII
200ChildST10ST767TE-G-SXT-S-AMaadA5, blaTEM-1B, dfrA17, mphA, strA, strB, sul1, sul2, tetAIncFIB(AP001918), IncFIA, IncFII(pCoo)FII10, FIA2, FIB20TE-G-SXT-S-AMFIB, FIA, A/C, FIIS, FII
202ChildST2952TE-G-SXT-S-AMblaTEM-1B, dfrA8, strA, strB, sul2, tetAIncQ1, IncFII(pCoo)FII16TE-G-AM-SXT-SFIIS, FII
203ChildST10ST2TE-G-SXT-S-AMblaTEM-1B, dfrA5, strA, strB, sul1, sul2, tetBIncFII(pRSB107), IncB/O/K/Z, IncI2FII6TE-G-SXT-S-AMeFIIS, FII
TE-G-S-AMeI2, L, FIIS, FIC, FII
212ChickenST394TE-G-SXT-S-AMaadA1, blaTEM-1B, dfrA1, strA, strB, sul1, sul2, tetAIncFII(pHN7A8), IncFII, Col(BS512)FII11TE-G-SXT-S-AMI1α, FIB, FIA, P, FIIS, FII
233ChildST131TE-G-SXT-S-AMblaTEM-1B, dfrA8, strA, strB, sul2, tetBIncFIB(AP001918), IncFII, IncFIC(FII), IncFIA, IncFII(pRSB107), IncI1, Col(BS512)FII1, FIA1, FIB1TE-G-SXT-S-AMeI2, FIIS, FIC, FII
TE-G-S-AMeFIIS, FIC, FII
71ChildSTNEW3TE-G-SXTaadA5, dfrA17, qnrB19, sul2, tetAp0111NEW1TE-G-SXTI1γ, FIIS, FII
253ChildST3075TE-G-SXTdfrA14, strA, strB, sul2, tetAIncFII(pCoo), IncY, IncB/O/K/ZFII43, FIB24TE-G-SXTB/O, I1γ, A/C, FIIS, FIC, FII
50Guinea pigST189STNEW5TE-G-SXT-S-CIP‡aadA24, dfrA14, dfrA15, strA, strB, sul1, sul2IncFIB(AP001918), IncI1, IncI2, Col156FII17TE-G-SXT-SI1α, FIB, P, FII
226ChickenST155TE-G-SXT-S‡dfrA14, qnrB19, strA, strB, sul2, tetAIncFII(29)FII29TE-G-SXT-SFIIS, FII
241ChildST10ST2TE-G-SXT-S‡dfrA5, qnrB19, strA, strB, sul1, sul2, tetAIncB/O/K/Z, Col(MG828)NEW2TE-G-SXT-SeP, K
TE-G-SeP, K
102ChildST1196TE-G-SXT-S-AM-CF-C-CIPaadA1, aadA2, blaTEM-1B, cmlA1, dfrA12, floR, fosA, lnuF, sul3, tetAIncFIB(AP001918), IncFII(29), p0111FII29, FIB1TE-G-SXT-S-AM-CF-C-CIPFIB, FIIS, FII
  • a AMR genes, plasmids, and pMLST were obtained from WGS.

  • b MLST profiles were obtained from https://cge.cbs.dtu.dk/services/MLST/.

  • c Profiles linked by the same symbol (*, †, or ‡) indicate that three antimicrobial resistance patterns, including isolates differing only in CIP, were considered a single multiresistance pattern because this resistance can generally be associated with chromosomal elements. However, in some isolates, the resistance was transferred into transconjugants. The antimicrobial compounds are abbreviated as follows: AMC, amoxicillin-clavulanate; AM, ampicillin; CTX, cefotaxime; CF, cephalothin; C, chloramphenicol; CIP, ciprofloxacin; S, streptomycin; G, sulfisoxazole; TE, tetracycline; SXT, trimethoprim-sulfamethoxazole.

  • d Most isolates transferred resistance patterns.

  • e Some isolates transferred complete and partial patterns.

  • f One isolate transferred only a partial pattern.

  • g Replicons were characterized from transconjugants.

  • h Result of replicon typing referred to donor strain, because we obtained only a partial transconjugant.

  • i Result of replicon typing referred to donor strain, because there were no transconjugants obtained.